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A DREaMR system to simplify combining mutations with rescue transgenes in Aedes aegypti.
Chen J, Luo J, Gurav AS, Chen Z, Wang Y, Montell C
Genetics. 2021 Nov 5;219(3). pii: 6368066. doi: 10.1093/genetics/iyab146. PubMed Article

Plasmids from Article

ID Plasmid Purpose
1822031xTRE DREaMR 3p3GFP1xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.
1822041xTRE DREaMR 3p3DsR1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue.
1822053xTRE DREaMR 3p3GFP3xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.
1822063xTRE DREaMR 3p3DsR3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescue
182207DmU6 3p3 GFP LgRNAFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Drosophila
182208AeU6 3p3 GFP LgRNAFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti

Antibodies from Article