Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Verification of extracellular vesicle-mediated functional mRNA delivery by RNA editing
Somiya M, Kuroda S
bioRxiv 2022.01.25.477620
Article

Plasmids from Article

ID Plasmid Purpose
180501pcDNA3.1-NlucMammalian expression of Nluc
180502pcDNA3.1-Nluc(W12stop)Mammalian expression of Nluc(W12stop)
180503pCMV-CD63-L7AeHuman CD63-L7Ae fusion protein
180504pCIneo-Nluc-2xC/D boxMammalian expression of Nluc-2xC/D box
180505pCIneo-Nluc(W12stop)-2xC/D boxMammalian expression of Nluc(W12stop)-2xC/D box
180506PasCas13b-gRNA-Nluc(W12stop)PasCas13b-gRNA targeting Nluc(W12stop) for RNA editing
180507PasCas13b-gRNA-EGFP(W58stop)PasCas13b-gRNA targeting EGFP(W58stop) for RNA editing
180508pcDNA3.1-EGFP(W58stop)-HiBiTMammalian expression of EGFP(W58stop)-HiBiT

Antibodies from Article