Komagataeibacter Tool Kit (KTK): A Modular Cloning System for Multigene Constructs and Programmed Protein Secretion from Cellulose Producing Bacteria.
Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 10.1021/acssynbio.1c00358. Epub 2021 Nov 12. PubMed Article
Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 10.1021/acssynbio.1c00358. Epub 2021 Nov 12. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180517 | KTK_001 | Empty backbone for creation of entry parts for the KTK, contains sfGFP dropout |
| 180518 | KTK_009 | Empty backbone for D1.1 cloning, contains LacZ dropout |
| 180519 | KTK_010 | Empty backbone for D1.2 cloning, contains LacZ dropout |
| 180520 | KTK_136 | Empty backbone for D1.1 cloning, contains sfGFP dropout |
| 180521 | KTK_137 | Empty backbone for D1.2 cloning, contains sfGFP dropout |
| 180522 | KTK_015 | Empty backbone for D2.2a cloning, contains LacZ dropout |
| 180523 | KTK_016 | Empty backbone for D2.2b cloning, contains LacZ dropout |
| 180524 | KTK_095 | dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly |
| 180525 | KTK_354 | KTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete. |
| 180526 | KTK_034 | Entry vector containing pLux promoter |
| 180527 | KTK_002 | Entry vector containing J23104 promoter |
| 180528 | KTK_032 | Entry vector containing pLac promoter |
| 180529 | KTK_247 | Entry vector containing pTac promoter |
| 180530 | KTK_042 | Entry vector containing J23100 anderson promoter |
| 180531 | KTK_043 | Entry vector containing J23101 anderson promoter |
| 180532 | KTK_044 | Entry vector containing J23117 anderson promoter |
| 180533 | KTK_218 | Entry vector containing K.rhaeticus native promoter pTtaC (200 bases upstream from gene start codon) |
| 180534 | KTK_219 | Entry vector containing K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon) |
| 180535 | KTK_220 | Entry vector containing K.rhaeticus native promoter pAacsAB4 (200 bases upstream from gene start codon) |
| 180536 | KTK_221 | Entry vector containing K.rhaeticus native promoter pKr0953 (200 bases upstream from gene start codon) |
| 180537 | KTK_222 | Entry vector containing K.rhaeticus native promoter pCyd (200 bases upstream from gene start codon) |
| 180538 | KTK_223 | Entry vector containing K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon) |
| 180539 | KTK_225 | Entry vector containing K.rhaeticus native promoter pBlh (200 bases upstream from gene start codon) |
| 180540 | KTK_113 | Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced |
| 180541 | KTK_003 | Entry vector containing RBS BBa_B0034 |
| 180542 | KTK_029 | Entry vector containing RBS BBa_B0035 |
| 180543 | KTK_045 | Entry vector containing RBS 30,000 a.u. designed strength |
| 180544 | KTK_046 | Entry vector containing RBS 250 a.u. designed strength |
| 180545 | KTK_033 | Entry vector containing LuxR |
| 180546 | KTK_251 | Entry vector containing mScarlet |
| 180547 | KTK_316 | Entry vector containing sfGFP |
| 180548 | KTK_344 | Entry vector containing cjBlue |
| 180549 | KTK_346 | Entry vector containing gfasPurple |
| 180550 | KTK_345 | Entry vector containing eforRed |
| 180551 | KTK_047 | Entry vector containing sfGFP in E1.3a format |
| 180552 | KTK_226 | Entry vector containing K.rhaeticus native promoter pRutB (200 bases upstream from gene start codon) |
| 180553 | KTK_252 | Entry vector containing mScarlet in E1.3b format |
| 180554 | KTK_110 | Entry vector containing 7xhis tag in E1.3b format |
| 180555 | KTK_005 | Entry vector containing Terminator sequence (Bba_B0015) |
| 180556 | KTK_031 | Entry vector containing Terminator - BBa_B0010 terminator |
| 180557 | KTK_035 | Entry vector containing L3S1P00 terminator |
| 180558 | KTK_036 | Entry vector containing L3S1P13 terminator |
| 180559 | KTK_037 | Entry vector containing L3S1P22 terminator |
| 180560 | KTK_038 | Entry vector containing L3S2P11 terminator |
| 180561 | KTK_039 | Entry vector containing L3S2P21 terminator |
| 180562 | KTK_040 | Entry vector containing L3S2P24 terminator |
| 180563 | KTK_007 | Entry vector containing Terminator sequence (Bba_B0015) |
| 180564 | KTK_048 | Entry vector containing L3S1P00 |
| 180565 | KTK_139 | Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2 |
| 180566 | KTK_324 | Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a |