A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae.
Shaw WM, Khalil AS, Ellis T
ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423. PubMed Article
Shaw WM, Khalil AS, Ellis T
ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180654 | pMYT001 | Encodes pZ3 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180655 | pMYT002 | Encodes pLacD as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180656 | pMYT003 | Encodes pLacE as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180657 | pMYT004 | Encodes pLacC as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180658 | pMYT005 | Encodes pLacFEC as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180660 | pMYT007 | Encodes pLacFBA1 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180661 | pMYT008 | Encodes pLacENO2 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180662 | pMYT009 | Encodes pLacSpTDH3 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180663 | pMYT010 | Encodes pTet2 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180664 | pMYT011 | Encodes pTet3 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180665 | pMYT012 | Encodes pTet4 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180666 | pMYT013 | Encodes pTet5 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180667 | pMYT014 | Encodes pTet6 as a Type 2 part to be used in the Dueber YTK and Ellis MYTK systems |
| 180668 | pMYT015 | Encodes pTet7 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |
| 180669 | pMYT016 | Encodes Z3EV as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems |
| 180670 | pMYT017 | Encodes LacI as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems |
| 180671 | pMYT018 | Encodes rtTA as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems |
| 180672 | pMYT019 | Encodes mScarlet-I as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems |
| 180673 | pMYT020 | Encodes mNeonGreen as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems |
| 180674 | pMYT021 | Encodes mTagBFP2 as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems |
| 180675 | pMYT022 | Encodes mScarlet-I as a Type 4a part to be used in the Dueber YTK and Ellis MYT systems |
| 180676 | pMYT023 | Encodes mNeonGreen as a Type 4a part to be used in the Dueber YTK and Ellis MYT systems |
| 180677 | pMYT024 | Encodes mTagBFP2 as a Type 4a part to be used in the Dueber YTK and Ellis MYT systems |
| 180678 | pMYT025 | Encodes tTDH3 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems |
| 180679 | pMYT026 | Encodes tTEF1 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems |
| 180680 | pMYT027 | Encodes tTDH2 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems |
| 180681 | pMYT028 | Encodes tPDC1 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems |
| 180682 | pMYT029 | Encodes URA3 as a yeast Vector selectable marker to be used in the Ellis MYT system |
| 180683 | pMYT030 | Encodes LEU2 as a yeast Vector selectable marker to be used in the Ellis MYT system |
| 180684 | pMYT031 | Encodes HIS3 as a yeast Vector selectable marker to be used in the Ellis MYT system |
| 180685 | pMYT032 | Encodes TRP1 as a yeast Vector selectable marker to be used in the Ellis MYT system |
| 180686 | pMYT033 | Encodes MET17 as a yeast Vector selectable marker to be used in the Ellis MYT system |
| 180687 | pMYT034 | Encodes LYS2 as a yeast Vector selectable marker to be used in the Ellis MYT system |
| 180688 | pMYT035 | Encodes KanR as a yeast Vector selectable marker to be used in the Ellis MYT system |
| 180689 | pMYT036 | Encodes NatR as a yeast Vector selectable marker to be used in the Ellis MYT system |
| 180690 | pMYT037 | Encodes HygR as a yeast Vector selectable marker to be used in the Ellis MYT system |
| 180691 | pMYT038 | Encodes ZeoR as a yeast Vector selectable marker to be used in the Ellis MYT system |
| 180692 | pMYT039 | Assembly cassette with ConLS and ConR1 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180693 | pMYT040 | Assembly cassette with ConL1 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180694 | pMYT041 | Assembly cassette with ConL1 and ConR2 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180695 | pMYT042 | Assembly cassette with ConL2 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180696 | pMYT043 | Assembly cassette with ConL2 and ConR3 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180697 | pMYT044 | Assembly cassette with ConL3 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180698 | pMYT045 | Assembly cassette with ConL3 and ConR4 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180699 | pMYT046 | Assembly cassette with ConL4 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180700 | pMYT047 | Assembly cassette with ConL4 and ConR5 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180701 | pMYT048 | Assembly cassette with ConL5 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180702 | pMYT049 | Assembly cassette with ConL5 and ConR6 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180703 | pMYT050 | Assembly cassette with ConL6 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180704 | pMYT051 | Assembly cassette with ConL6 and ConR7 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180705 | pMYT052 | Assembly cassette with ConL7 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180706 | pMYT053 | Assembly cassette with ConL7 and ConR8 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180707 | pMYT054 | Assembly cassette with ConL8 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180708 | pMYT055 | Assembly cassette with ConL8 and ConR9 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180709 | pMYT056 | Assembly cassette with ConL9 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180710 | pMYT057 | Spacer cassette with ConLS and ConR1 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180711 | pMYT058 | Spacer cassette with ConL1 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180712 | pMYT059 | Spacer cassette with ConL1 and ConR2 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180713 | pMYT060 | Spacer cassette with ConL2 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180714 | pMYT061 | Spacer cassette with ConL2 and ConR3 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180715 | pMYT062 | Spacer cassette with ConL3 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180716 | pMYT063 | Spacer cassette with ConL3 and ConR4 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180717 | pMYT064 | Spacer cassette with ConL4 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180718 | pMYT065 | Spacer cassette with ConL4 and ConR5 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180719 | pMYT066 | Spacer cassette with ConL5 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180720 | pMYT067 | Spacer cassette with ConL5 and ConR6 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180721 | pMYT068 | Spacer cassette with ConL6 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180722 | pMYT069 | Spacer cassette with ConL6 and ConR7 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180723 | pMYT070 | Spacer cassette with ConL7 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180724 | pMYT071 | Spacer cassette with ConL7 and ConR8 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180725 | pMYT072 | Spacer cassette with ConL8 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180726 | pMYT073 | Spacer cassette with ConL8 and ConR9 connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180727 | pMYT074 | Spacer cassette with ConL9 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems |
| 180728 | pMYT075 | Markerless yeast integration Vector at Int.1 locus to used in the Dueber YTK and Ellis MYT systems |
| 180729 | pMYT076 | Markerless yeast integration Vector at Int.2 locus to used in the Dueber YTK and Ellis MYT systems |
| 180730 | pMYT077 | Markerless yeast integration Vector at Int.3 locus to used in the Dueber YTK and Ellis MYT systems |
| 180731 | pMYT078 | Markerless yeast integration Vector at Int.4 locus to used in the Dueber YTK and Ellis MYT systems |
| 180732 | pMYT079 | Markerless yeast integration Vector at Int.5 locus to used in the Dueber YTK and Ellis MYT systems |
| 180733 | pMYT080 | Markerless yeast integration Vector at Int.6 locus to used in the Dueber YTK and Ellis MYT systems |
| 180734 | pMYT081 | Markerless yeast integration Vector at Int.7 locus to used in the Dueber YTK and Ellis MYT systems |
| 180736 | pMYT083 | Markerless yeast integration Vector at Int.9 locus to used in the Dueber YTK and Ellis MYT systems |
| 180737 | pMYT084 | Markerless yeast integration Vector at Int.10 locus to used in the Dueber YTK and Ellis MYT systems |
| 180738 | pMYT085 | Markerless yeast integration Spacer at Int.1 locus to used in the Ellis MYT system |
| 180739 | pMYT086 | Markerless yeast integration Spacer at Int.2 locus to used in the Ellis MYT system |
| 180740 | pMYT087 | Markerless yeast integration Spacer at Int.3 locus to used in the Ellis MYT system |
| 180741 | pMYT088 | Markerless yeast integration Spacer at Int.4 locus to used in the Ellis MYT system |
| 180742 | pMYT089 | Markerless yeast integration Spacer at Int.5 locus to used in the Ellis MYT system |
| 180743 | pMYT090 | Markerless yeast integration Spacer at Int.6 locus to used in the Ellis MYT system |
| 180744 | pMYT091 | Markerless yeast integration Spacer at Int.7 locus to used in the Ellis MYT system |
| 180746 | pMYT093 | Markerless yeast integration Spacer at Int.9 locus to used in the Ellis MYT system |
| 180747 | pMYT094 | Markerless yeast integration Spacer at Int.10 locus to used in the Ellis MYT system |
| 198864 | pMYT082 | Markerless yeast integration Vector at Int.8 locus to be used in the Dueber YTK and Ellis MYT systems |
| 198865 | pMYT092 | Markerless yeast integration Spacer at Int.8 locus to be used in the Ellis MYT system |
| 198866 | pMYT095 | CRISPR/Cas9 Vector with URA3 selectable marker to be used in the Ellis MYT system |
| 198867 | pMYT096 | Transient CRISPR/Cas9 Vector without a selectable marker to be used in the Ellis MYT system |
| 203322 | pMYT006 | Encodes pLacHTA1 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems |