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A genome-scale screen for synthetic drivers of T cell proliferation.
Legut M, Gajic Z, Guarino M, Daniloski Z, Rahman JA, Xue X, Lu C, Lu L, Mimitou EP, Hao S, Davoli T, Diefenbach C, Smibert P, Sanjana NE
Nature. 2022 Mar;603(7902):728-735. doi: 10.1038/s41586-022-04494-7. Epub 2022 Mar 16. PubMed Article

Plasmids from Article

ID Plasmid Purpose
181970pOT_1 - lenti-EFS-LTBR-2A-puroExpresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.
181971pOT_2 - lenti-EFS-tNGFR-2A-puroExpresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.
181972pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBRExpresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.
181973pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBRExpresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.
181974pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFRExpresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
181975pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFRExpresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

Antibodies from Article