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Live-Cell Fluorescence Lifetime Multiplexing Using Synthetic Fluorescent Probes.
Frei MS, Koch B, Hiblot J, Johnsson K
ACS Chem Biol. 2022 Jun 17;17(6):1321-1327. doi: 10.1021/acschembio.2c00041. Epub 2022 May 18. PubMed Article

Plasmids from Article

ID Plasmid Purpose
181998pcDNA5/FRT/TO_H2B-STfCMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell lines
181999pcDNA5/FRT/TO_STf_LamB1CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines
182000pcDNA5/FRT/TO_STfCMV overexpression of SNAP-tag fast in mammalian cell lines
182001pcDNA5/FRT/TO_NES_STfCMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines
182002pcDNA5/FRT/TO_TOMM20-STfCMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell lines
182003pcDNA5/FRT/TO_[Cox8a]x2_STfCMV overexpression of SNAP-tag fast in the mitochondrial matrix of mammalian cell lines
182004pcDNA5/FRT/TO_CalR_STf_KDELCMV overexpression of SNAP-tag fast in the endoplasmic reticulum of mammalian cell lines
182005pcDNA5/FRT/TO_b4g-STfCMV overexpression of SNAP-tag fast in the golgi apparatus of mammalian cell lines
182006pcDNA5/FRT/TO_LAMP1_STfCMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines
182007pcDNA5/FRT/TO_STf_SKLCMV overexpression of SNAP-tag fast in the peroxisome of mammalian cell lines
182008pcDNA5/FRT/TO_Lyn11-STfCMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines
182009pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmbCMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines
182010pcDNA5/FRT/TO_Cep41-STfCMV overexpression of SNAP-tag fast in mammalian cell lines associated to microtubules
182011pcDNA5/FRT/TO_Lifeact_STfCMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin
182012pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDELCMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell lines

Antibodies from Article