Improving genomically recoded Escherichia coli to produce proteins containing non-canonical amino acids.
Perez JG, Carlson ED, Weisser O, Kofman C, Seki K, Des Soye BJ, Karim AS, Jewett MC
Biotechnol J. 2021 Dec 11:e2100330. doi: 10.1002/biot.202100330. PubMed Article
Perez JG, Carlson ED, Weisser O, Kofman C, Seki K, Des Soye BJ, Karim AS, Jewett MC
Biotechnol J. 2021 Dec 11:e2100330. doi: 10.1002/biot.202100330. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182773 | C321.∆A ompT- rne- lon- | Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, lon inactivated |
| 182774 | C321.∆A ompT- rne- endA- | Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA inactivated |
| 182775 | C321.∆A ompT- rne- endA- lon- | Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated |
| 182776 | C321.∆A T7RNAP | Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted |
| 182777 | C321.∆A T7RNAP ompT- rne- | Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated |
| 182778 | C321.∆A T7RNAP ompT- rne- lon- | Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated |