Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
A Suite of New Strain Construction Vectors for Gene Expression Knockdown in Budding Yeast.
Shively CA, Dong F, Mitra RD
ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547. PubMed Article

A subset of these plasmids is included in the CEAS (C-SWAT for Essential Allele Strains) Kit.

Plasmids from Article

ID Plasmid Purpose
182509C-SWAT 6FLAGC-SWAT compatible vector for C-terminal tagging with 6 FLAG
182510C-SWAT GFPC-SWAT compatible vector for C-terminal tagging with GFP
182511C-SWAT GFP DAmPC-SWAT compatible vector for C-terminal tagging with GFP DAmP. GFP is immediately followed by the Hygromycin B (Hph) resistance cassette.
182512C-SWAT GFP CL1C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.
182513C-SWAT GFP CL1 DAmPC-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.
182514C-SWAT AID*-GFP osTIR1C-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.
182515C-SWAT AID*-6FLAG osTIR1C-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.
182516C-SWAT IAA17-6FLAGC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.
182517C-SWAT IAA17-3HAC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3HA. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.
182518C-SWAT IAA17-3MYCC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3MYC. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.
182519Gal1/10p CAS9 Gal1/10p I-SceIGalactose-Induced Expression vector for Cas9 and I-SceI.
182520SIR4 gRNA HISExpression vector for gRNA directed against SIR4 ORF.
182521CAN1 gRNA HISExpression vector for gRNA directed against CAN1 ORF.
182522C-SWAT IAA17-6FLAGC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 with CAN1 homology arms for genome integration.
182523C-SWAT SMAShC-SWAT compatible vector for C-terminal tagging with SMASh Degron. (Protease Cleavage Site: DEMEECSKHL).
182524C-SWAT 3MYC SMAShC-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSKHL).
182525C-SWAT 3MYC SMAShC-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSQHL).
182526NAT-TET-OFF -MET - 3xHADoxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.
182527NAT-TET-OFF -LEU- 4xFLAGDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
193314NAT-TET-OFF – ubiquitin – LEU – 4MYCDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
193315HYG-TET-OFF – ubiquitin – LEU – 4FLAGDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
193316HYG-TET-OFF – ubiquitin – LEU – 4MYCDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
193317KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAGDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
193318KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYCDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
193319C-SWAT IAA17C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron). ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.
193320C-SWAT AID*-6MYC osTIR1C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.
193321C-SWAT Compatible pAIDA2(6FLAG)C-SWAT compatible vector for C-terminal tagging with TAP-AID*-6FLAG. osTIR1 is carried downstream of the C-terminal tag.
193322C-SWAT IAA17-6FLAG (no NLS)C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

Antibodies from Article