Inducible expression of large gRNA arrays for multiplexed CRISPRai applications.
Shaw WM, Studena L, Roy K, Hapeta P, McCarty NS, Graham AE, Ellis T, Ledesma-Amaro R
Nat Commun. 2022 Aug 25;13(1):4984. doi: 10.1038/s41467-022-32603-7. PubMed Article
Shaw WM, Studena L, Roy K, Hapeta P, McCarty NS, Graham AE, Ellis T, Ledesma-Amaro R
Nat Commun. 2022 Aug 25;13(1):4984. doi: 10.1038/s41467-022-32603-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182827 | pWS3799-gRNA-Csy4-template | SpCas9 gRNA-Csy4 template |
| 182828 | pWS5321-CRISPRai-Markerless | Saccharomyces cerevisiae inducible CRISPRai vector - markerless |
| 182829 | pWS5390-CRISPRai-URA3 | Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker |
| 182830 | pWS5391-CRISPRai-LEU2 | Saccharomyces cerevisiae inducible CRISPRai vector - LEU2 marker |
| 182831 | pWS5392-CRISPRai-HIS3 | Saccharomyces cerevisiae inducible CRISPRai vector - HIS3 marker |
| 182832 | pWS5393-CRISPRai-TRP1 | Saccharomyces cerevisiae inducible CRISPRai vector - TRP1 marker |
| 182833 | pWS5394-CRISPRai-MET17 | Saccharomyces cerevisiae inducible CRISPRai vector - MET17 marker |
| 182834 | pWS5395-CRISPRai-LYS2 | Saccharomyces cerevisiae inducible CRISPRai vector - LYS2 marker |
| 182835 | pWS5396-CRISPRai-KanR | Saccharomyces cerevisiae inducible CRISPRai vector - KanR marker |
| 182836 | pWS5397-CRISPRai-NatR | Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker |
| 182837 | pWS5398-CRISPRai-HygR | Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker |
| 182838 | pWS5399-CRISPRai-ZeoR | Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker |
| 185742 | pWS3865 | Sub-array backbone 1/4 |
| 185743 | pWS3866 | Sub-array backbone 2/4 |
| 185744 | pWS3867 | Sub-array backbone 3/4 |
| 185745 | pWS3868 | Sub-array backbone 4/4 |
| 185746 | pWS3805 | Sub-array spacer 1/4 |
| 185747 | pWS3806 | Sub-array spacer 2/4 |
| 185748 | pWS3807 | Sub-array spacer 3/4 |
| 185749 | pWS3808 | Sub-array spacer 4/4 |