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Chromosome clustering by Ki-67 excludes cytoplasm during nuclear assembly.
Cuylen-Haering S, Petrovic M, Hernandez-Armendariz A, Schneider MWG, Samwer M, Blaukopf C, Holt LJ, Gerlich DW
Nature. 2020 Nov;587(7833):285-290. doi: 10.1038/s41586-020-2672-3. Epub 2020 Sep 2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
182918H2B-mNeonGreen_FRB(DmrC)_IRESpuro2Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B
182921EGFP-Ki-67(RASA)-mCherry_IRESpuro2Expresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.

Antibodies from Article