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Multiplexed bioluminescence microscopy via phasor analysis.
Yao Z, Brennan CK, Scipioni L, Chen H, Ng KK, Tedeschi G, Parag-Sharma K, Amelio AL, Gratton E, Digman MA, Prescher JA
Nat Methods. 2022 Jul;19(7):893-898. doi: 10.1038/s41592-022-01529-9. Epub 2022 Jun 23. PubMed Article

Plasmids from Article

ID Plasmid Purpose
183036pLenti_CMV:Flag-CeNLuc_SV40:PuroRExpresses the FLAG-tagged CeNLuc (Blue LumiFluor) in mammalian cells.
183037pLenti_CMV:Flag-GpNLuc_SV40:PuroRExpresses the FLAG-tagged GpNLuc (Green LumiFluor) in mammalian cells.
183038pLenti_CMV:Flag-OgNLuc_SV40:PuroRExpresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.
183039pLenti_CMV:Flag-CeNL_SV40:PuroRExpresses the FLAG-tagged CeNL (Blue Nanolantern) in mammalian cells.
183040pLenti_CMV:Flag-GeNL_SV40:PuroRExpresses the FLAG-tagged GeNL (Green Nanolantern) in mammalian cells.
183041pLenti_CMV:Flag-YeNL_SV40:PuroRExpresses the FLAG-tagged YeNL (Yellow Nanolantern) in mammalian cells.
183042pLenti_CMV:Flag-OeNL_SV40:PuroRExpresses the FLAG-tagged OeNL (Orange Nanolantern) in mammalian cells.
183044pLenti_CMV:Flag-LumiScarlet_SV40:PuroRExpresses FLAG-tagged LumiScarlet in mammalian cells.
183045pLenti_CMV:Flag-NLuc_SV40:PuroRExpresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.
183046pLenti_CMV:Flag-dKeNLuc_SV40:PuroRExpresses the FLAG-tagged dKeNLuc (Far-Red dKeima LumiFluor) in mammalian cells.
183047pLenti_CMV:Flag-KaNLuc_SV40:PuroRExpresses the FLAG-tagged KaNLuc (Far-Red LSSmKate2 LumiFluor) in mammalian cells.
183048pLenti_CMV: Flag-Akaluc_SV40:PuroRExpresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.
183049pLenti_CMV: Flag-Antares_SV40:PuroRExpresses FLAG-tagged Antares in mammalian cells.

Antibodies from Article