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All-in-one: A Robust Fluorescent Fusion Protein Vector Toolbox for Protein Localization and BiFC Analyses in Plants.
Han J, Ma K, Li H, Su J, Zhou L, Tang J, Zhang S, Hou Y, Chen L, Liu YG, Zhu Q
Plant Biotechnol J. 2022 Feb 18. doi: 10.1111/pbi.13790. PubMed Article

Plasmids from Article

ID Plasmid Purpose
183151pYL322d1/N-eGFPPlant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.
183152pYL322d1/eGFP-CPlant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP.
183153pYL322d1/N-VnBimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.
183154pYL322d1/Vn-CBimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.
183155pUC19/N-eCFPPlant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFP
183156pUC19/eCFP-CPlant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFP
183157pUC19/N-VcBimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.
183158pUC19/Vc-CBimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.
183159pUC19/N-mChePlant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry.
183160pUC19/mChe-CPlant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.
183161pUC19/mCheConstruct for transient expression of cell nucleus-, cytoplasm-targeted mCherry in plants.
183162pUC19/NLS-mCheConstruct for transient expression of cell nucleus-targeted mCherry in plants.
183163pUC19/ER-mCheConstruct for transient expression of endoplasmic reticulum-targeted mCherry in plants.
183164pUC19/Pt-mCheConstruct for transient expression of plastids-targeted mCherry in plants.
183165pUC19/Tp-mCheConstruct for transient expression of tonoplast-targeted mCherry in plants.
183166pUC19/CF-mCheConstruct for transient expression of cytoplasmic foci-targeted mCherry in plants.
183167pUC19/Mito-mCheConstruct for transient expression of mitochondrion-targeted mCherry in plants.
183168pUC19/mChe-PMConstruct for transient expression of plasma membrane-targeted mCherry in plants.
183169pUC19/mChe-GolgiConstruct for transient expression of Golgi apparatus-targeted mCherry in plants.
183170pUC19/mChe-PxConstruct for transient expression of peroxisomes-targeted mCherry in plants.
183171pUC19/mChe-CsConstruct for transient expression of cytoskeleton-targeted mCherry in plants.
183172pUC19/Pd-mCheConstruct for transient expression of plasmodesmata-targeted mCherry in plants.
183173pYL1300UaUfPlant binary vector with ccdB flanked by Ua and Uf unique nucleotide sequences.

Antibodies from Article