Evolution-inspired redesign of the LPS receptor caspase-4 into an interleukin-1beta converting enzyme.
Devant P, Cao A, Kagan JC
Sci Immunol. 2021 Aug 10;6(62). doi: 10.1126/sciimmunol.abh3567. PubMed Article
Devant P, Cao A, Kagan JC
Sci Immunol. 2021 Aug 10;6(62). doi: 10.1126/sciimmunol.abh3567. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183356 | pMSCV-IRES-GFP-human-Caspase-4 | Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183357 | pMSCV-IRES-GFP-redesigned-human-Caspase-4_ANR261ENP_D315H_M318K_T352K | Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183358 | pMSCV-IRES-GFP-mouse-Caspase-11 | Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183359 | pMSCV-IRES-GFP-human-Caspase-1 | Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183360 | pMSCV-IRES-GFP-human-Caspase-1-H340D | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183361 | pMSCV-IRES-GFP-mouse-Caspase-1 | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183362 | pMSCV-IRES-GFP-mouse-Caspase-1-H340D | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183363 | pMSCV-IRES-GFP-dog-Caspase-1/4a | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183364 | pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183365 | pMSCV-IRES-GFP-dog-Caspase-1/4b | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183366 | pMSCV-IRES-GFP-cat-Caspase-1/4b | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183367 | pMSCV-IRES-GFP-fox-Caspase-1/4b | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183368 | pMSCV-IRES-GFP-harbour-seal-Caspase-1/4b | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183369 | pMSCV-IRES-GFP-dingo-Caspase-1/4b | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183370 | pMSCV-IRES-GFP-polar-bear-Caspase-1/4b | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183371 | pMSCV-IRES-GFP-panda-Caspase-1/4b | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183372 | pMSCV-IRES-GFP-tiger-Caspase-1/4b | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183373 | pMSCV-IRES-GFP-cheetah-Caspase-1/4b | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183374 | pMSCV-IRES-GFP-lemur-Caspase-4 | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183375 | pMSCV-IRES-GFP-rabbit-Caspase-4 | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183376 | pMSCV-IRES-GFP-sheep-Caspase-4 | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183377 | pMSCV-IRES-GFP-bat-Caspase-4 | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183378 | pMSCV-IRES-GFP-manatee-Caspase-4 | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183379 | pMSCV-IRES-GFP-wombat-Caspase-4 | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183380 | pMSCV-IRES-GFP-horse-Caspase-4 | Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183381 | pET28_human-Caspase-4_enzymatic-domain | Overexpression of catalytic domain of inflammatory caspase in bacteria |
| 183382 | pET28_mouse-Caspase-11_enzymatic-domain | Overexpression of catalytic domain of inflammatory caspase in bacteria |
| 183383 | pET28_canine-Caspase-1/4_enzymatic-domain | Overexpression of catalytic domain of inflammatory caspase in bacteria |
| 183385 | pET28_mouse-Caspase-1_enzymatic-domain | Overexpression of catalytic domain of inflammatory caspase in bacteria |
| 183386 | pET28_mouse-pro-IL1B | Overexpression of catalytic domain of inflammatory caspase in bacteria |
| 183387 | pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain | Overexpression of catalytic domain of inflammatory caspase in bacteria |
| 183388 | pET28_mouse-Caspase-1-H340D_enzymatic-domain | Overexpression of catalytic domain of inflammatory caspase in bacteria |
| 183389 | pET28_mouse-pro-IL18 | Overexpression of catalytic domain of inflammatory caspase in bacteria |
| 183390 | pET28_human-pro-IL18 | Overexpression of catalytic domain of inflammatory caspase in bacteria |
| 183391 | pMSCV-IRES-GFP-Mouse-caspase-1/11a | Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183392 | pMSCV-IRES-GFP-Mouse-caspase-1/11b | Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183393 | pMSCV-IRES-GFP-human-caspase-1/4a | Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183394 | pMSCV-IRES-GFP-human-caspase-1/4b | Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183395 | pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+human-Caspase-4-catalytic domain | Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183396 | pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+mouse-Caspase-11-catalytic domain | Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183397 | pMSCV-IRES-GFP-Hybrid-caspase_human-caspase-4-CARD+canine-Caspase-1/4-catalytic domain | Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |
| 183398 | pMSCV-IRES-GFP-Hybrid-caspase_mouse-caspase-11-CARD+canine-Caspase-1/4-catalytic domain | Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction |