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Kinase-independent synthesis of 3-phosphorylated phosphoinositides by a phosphotransferase.
Walpole GFW, Pacheco J, Chauhan N, Clark J, Anderson KE, Abbas YM, Brabant-Kirwan D, Montano-Rendon F, Liu Z, Zhu H, Brumell JH, Deiters A, Stephens LR, Hawkins PT, Hammond GRV, Grinstein S, Fairn GD
Nat Cell Biol. 2022 Apr 28. pii: 10.1038/s41556-022-00895-y. doi: 10.1038/s41556-022-00895-y.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
183651NES-EGFP-PH-BTKx1PtdIns(3,4,5)P3 biosensor
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183652NES-EGFP-PH-BTKx2PtdIns(3,4,5)P3 biosensor
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183653NES-mCherry-PH-BTKx1PtdIns(3,4,5)P3 biosensor
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183654NES-mCherry-PH-BTKx2PtdIns(3,4,5)P3 biosensor
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183655SopB-EGFPC-terminally tagged Salmonella Typhimurium SopB for mammalian expression
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183656SopB(C460S)-EGFPC-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression
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183657EGFP-SopBN-terminally tagged Salmonella Typhimurium SopB for mammalian expression
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183658EGFP-SopB(C460S)N-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression
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183659EGFP-SopB(K525A)N-terminally tagged Salmonella Typhimurium SopB (K525A) for mammalian expression
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183660EGFP-SopB(K528A)N-terminally tagged Salmonella Typhimurium SopB (K528A) for mammalian expression
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183661EGFP-SopB(33-554)N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression
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183662EGFP-SopB(33-520)N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression
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183663EGFP-SopB(68-554)N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression
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183664EGFP-SopB(172-554)N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression
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183665EGFP-SopB(211-554)N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression
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183666EGFP-IpgDN-terminally tagged Shigella flexneri IpgD for mammalian expression
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183667EGFP-IpgD(C439S)N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression
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183668pUG36-NES-EGFP-cPHx3PtdIns(3,4)P2 biosensor for expression in yeast
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183669pUG34-NES-EGFP-cPHx3PtdIns(3,4)P2 biosensor for expression in yeast
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183670pESC-LEU-SopBInducible Salmonella Typhimurium SopB for expression in yeast
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183671pESC-Leu-SopB(C460S)Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast
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183672pESC-LEU-IpgDInducible Shigella flexneri IpgD for expression in yeast
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183673pESC-LEU-IpgD(C439S)Inducible Shigella flexneri IpgD (catalytically inactive) for expression in yeast
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183674His6-EGFP-cPHx1PtdIns(3,4)P2 biosensor for purification from bacteria
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183675His6-EGFP-PH-PLCD1PtdIns(4,5)P2 biosensor for purification from bacteria
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183676His6-SMT3-SopBRecombinant Salmonella Typhimurium SopB
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183677His6-SMT3-SopB(C460S)Recombinant Salmonella Typhimurium SopB (catalytically inactive)
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183678mRFP-FKBP-INPP5E(D556A)Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)
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Antibodies from Article