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Addgene
Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron.
Teng IT, Nazzari AF, Choe M, Liu T, Oliveira de Souza M, Petrova Y, Tsybovsky Y, Wang S, Zhang B, Artamonov M, Madan B, Huang A, Lopez Acevedo SN, Pan X, Ruckwardt TJ, DeKosky BJ, Mascola JR, Misasi J, Sullivan NJ, Zhou T, Kwong PD
PLoS One. 2022 May 24;17(5):e0268767. doi: 10.1371/journal.pone.0268767. eCollection 2022. PubMed Article

Plasmids from Article

ID Plasmid Purpose
176324pVRC8400-SARS-CoV-2-S2P-D614G-AVIExpresses SARS-CoV-2 S2P protein containing D614G mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
176325pVRC8400-SARS-CoV-2-S2P-B.1.1.7-AVIExpresses SARS-CoV-2 S2P protein from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176326pVRC8400-SARS-CoV-2-S2P-B.1.351-AVIExpresses SARS-CoV-2 S2P protein from the B.1.351 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176327pVRC8400-SARS-CoV-2-S2P-P.1-AVIExpresses SARS-CoV-2 S2P protein from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176328pVRC8400-SARS-CoV-2-S2P-B.1.429-AVIExpresses SARS-CoV-2 S2P protein from the B.1.429 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176329pVRC8400-SARS-CoV-2-S2P-B.1.526-S477N-AVIExpresses SARS-CoV-2 S2P protein from the B.1.526 variant containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
176330pVRC8400-SARS-CoV-2-S2P-B.1.526-E484K-AVIExpresses SARS-CoV-2 S2P protein from the B.1.526 variant containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
176331pVRC8400-SARS-CoV-2-S2P-B.1.617-E154K-L452R-E484Q-D614G-P681R-AVIExpresses SARS-CoV-2 S2P protein from the B.1.617 variant containing E154K-L452R-E484Q-D614G-P681R mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176332pVRC8400-SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D-AVIExpresses SARS-CoV-2 S2P protein from the B.1.617 variant containing G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176333pVRC8400-SARS-CoV-2-S2P-B.1.617.1-AVIExpresses SARS-CoV-2 S2P protein from the B.1.617.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176334pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVIExpresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176335pVRC8400-SARS-CoV-2-S2P-AY.1-AVIExpresses SARS-CoV-2 S2P protein from the AY.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176336pVRC8400-SARS-CoV-2-S2P-B.1.618-AVIExpresses SARS-CoV-2 S2P protein from the B.1.618 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176424pVRC8400-SARS-CoV-2-NTD-B.1.1.7-AVIExpresses SARS-CoV-2 NTD domain from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176426pVRC8400-SARS-CoV-2-NTD-B.1.351-AVIExpresses SARS-CoV-2 NTD domain from the B.1.351 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176429pVRC8400-SARS-CoV-2-NTD-P.1-AVIExpresses SARS-CoV-2 NTD domain from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176431pVRC8400-SARS-CoV-2-NTD-W152C-AVIExpresses SARS-CoV-2 NTD domain containing W152C mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
176432pVRC8400-SARS-CoV-2-NTD-T95I-D253G-AVIExpresses SARS-CoV-2 NTD domain containing T95I-D253G mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176433pVRC8400-SARS-CoV-2-NTD-E154K-AVIExpresses SARS-CoV-2 NTD domain containing E154K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
176434pVRC8400-SARS-CoV-2-NTD-G142D-E154K-AVIExpresses SARS-CoV-2 NTD domain containing G142D-E154K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176435pVRC8400-SARS-CoV-2-NTD-B.1.617.1-AVIExpresses SARS-CoV-2 NTD domain from the B.1.617.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176436pVRC8400-SARS-CoV-2-NTD-B.1.617.2-AVIExpresses SARS-CoV-2 NTD domain from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176437pVRC8400-SARS-CoV-2-NTD-AY.1-AVIExpresses SARS-CoV-2 NTD domain from the AY.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176438pVRC8400-SARS-CoV-2-NTD-B.1.618-AVIExpresses SARS-CoV-2 NTD domain from the B.1.618 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
176439pVRC8400-SARS-CoV-2-RBD-N501Y-AVI Expresses SARS-CoV-2 RBD domain containing N501Y mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
176440pVRC8400-SARS-CoV-2-RBD-K417N-E484K-N501Y-AVI Expresses SARS-CoV-2 RBD domain containing K417N-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176441pVRC8400-SARS-CoV-2-RBD-K417T-E484K-N501Y-AVI Expresses SARS-CoV-2 RBD domain containing K417T-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176442pVRC8400-SARS-CoV-2-RBD-L452R-AVI Expresses SARS-CoV-2 RBD domain containing L452R mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
176443pVRC8400-SARS-CoV-2-RBD-S477N-AVI Expresses SARS-CoV-2 RBD domain containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
176444pVRC8400-SARS-CoV-2-RBD-E484K-AVI Expresses SARS-CoV-2 RBD domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
176445pVRC8400-SARS-CoV-2-RBD-L452R-E484Q-AVI Expresses SARS-CoV-2 RBD domain containing L452R-E484Q mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176446pVRC8400-SARS-CoV-2-RBD-L452R-T478K-AVI Expresses SARS-CoV-2 RBD domain containing L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176447pVRC8400-SARS-CoV-2-RBD-K417N-L452R-T478K-AVI Expresses SARS-CoV-2 RBD domain containing K417N-L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176448pVRC8400-SARS-CoV-2-RBD-SD1-WA1-AVI Expresses SARS-CoV-2 RBD-SD1 domain from the WA1 strain with single chain Fc, HRV3C protease cleavage site, and AVI tag
176449pVRC8400-SARS-CoV-2-RBD-SD1-N501Y-A570D-AVI Expresses SARS-CoV-2 RBD-SD1 domain containing N501Y-A570D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176450pVRC8400-SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y-AVIExpresses SARS-CoV-2 RBD-SD1 domain containing K417N-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176451pVRC8400-SARS-CoV-2-RBD-SD1-K417T-E484K-N501Y-AVI Expresses SARS-CoV-2 RBD-SD1 domain containing K417T-E484K-N501Y mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176452pVRC8400-SARS-CoV-2-RBD-SD1-L452R-AVI Expresses SARS-CoV-2 RBD-SD1 domain containing L452R mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
176453pVRC8400-SARS-CoV-2-RBD-SD1-S477N-AVI Expresses SARS-CoV-2 RBD-SD1 domain containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
176454pVRC8400-SARS-CoV-2-RBD-SD1-E484K-AVI Expresses SARS-CoV-2 RBD-SD1 domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag
176455pVRC8400-SARS-CoV-2-RBD-SD1-L452R-E484Q-AVI Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-E484Q mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176456pVRC8400-SARS-CoV-2-RBD-SD1-L452R-T478K-AVI Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
176457pVRC8400-SARS-CoV-2-RBD-SD1-K417N-L452R-T478K-AVI Expresses SARS-CoV-2 RBD-SD1 domain containing K417N-L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
181693pVRC8400-SARS-CoV-2-S2P-C.37-AVIExpresses SARS-CoV-2 S2P protein from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
181694pVRC8400-SARS-CoV-2-S2P-B.1.621-AVIExpresses SARS-CoV-2 S2P protein from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
181695pVRC8400-SARS-CoV-2-S2P-B.1.1.529-AVIExpresses SARS-CoV-2 S2P protein from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
181696pVRC8400-SARS-CoV-2-NTD-C.37-AVIExpresses SARS-CoV-2 NTD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
181697pVRC8400-SARS-CoV-2-NTD-B.1.621-AVIExpresses SARS-CoV-2 NTD domain from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
181698pVRC8400-SARS-CoV-2-NTD-B.1.1.529-AVIExpresses SARS-CoV-2 NTD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
181699pVRC8400-SARS-CoV-2-RBD-C.37-AVIExpresses SARS-CoV-2 RBD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
181700pVRC8400-SARS-CoV-2-RBD-B.1.621-AVIExpresses SARS-CoV-2 RBD domain from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
181701pVRC8400-SARS-CoV-2-RBD(aa335-526)-B.1.1.529-AVIExpresses SARS-CoV-2 RBD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
181702pVRC8400-SARS-CoV-2-RBD-SD1-C.37-AVIExpresses SARS-CoV-2 RBD-SD1 domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
181703pVRC8400-SARS-CoV-2-RBD-SD1-B.1.621-AVIExpresses SARS-CoV-2 RBD-SD1 domain from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
181704pVRC8400-SARS-CoV-2-RBD-SD1-B.1.1.529-AVIExpresses SARS-CoV-2 RBD-SD1 domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
182930pVRC8400-SARS-CoV-2-RBD(aa330-526)-B.1.1.529-AVIExpresses SARS-CoV-2 RBD (aa324-526) domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
184531pVRC8400-SARS-CoV-2-S2P-BA.2-AVIExpresses SARS-CoV-2 S2P protein from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
184532pVRC8400-SARS-CoV-2-NTD-BA.2-AVIExpresses SARS-CoV-2 NTD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
184533pVRC8400-SARS-CoV-2-RBD-BA.2-AVIExpresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
184534pVRC8400-SARS-CoV-2-RBD-SD1-BA.2-AVIExpresses SARS-CoV-2 RBD-SD1 domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
196617pVRC8400-SARS-CoV-2-RBD-BJ.1-AVIExpresses SARS-CoV-2 RBD protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
196618pVRC8400-SARS-CoV-2-RBD-BQ.1.1-AVIExpresses SARS-CoV-2 RBD protein from the BQ.1.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
196619pVRC8400-SARS-CoV-2-RBD-XBB-AVIExpresses SARS-CoV-2 RBD protein from the XBB variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
196620pVRC8400-SARS-CoV-2-S2P-BJ.1-AVIExpresses SARS-CoV-2 S2P protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
196621pVRC8400-SARS-CoV-2-S2P-BQ.1.1-AVIExpresses SARS-CoV-2 S2P protein from the BQ.1.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
196622pVRC8400-SARS-CoV-2-S2P-XBB-AVIExpresses SARS-CoV-2 S2P protein from the XBB variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
198465pVRC8400-SARS-CoV-2-S2P-XBB.1.5-AVIExpresses SARS-CoV-2 S2P protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
198466pVRC8400-SARS-CoV-2-RBD-XBB.1.5-AVIExpresses SARS-CoV-2 RBD protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Antibodies from Article