Targeting stem-loop 1 of the SARS-CoV-2 5' UTR to suppress viral translation and Nsp1 evasion.
Vora SM, Fontana P, Mao T, Leger V, Zhang Y, Fu TM, Lieberman J, Gehrke L, Shi M, Wang L, Iwasaki A, Wu H
Proc Natl Acad Sci U S A. 2022 Mar 1;119(9). pii: 2117198119. doi: 10.1073/pnas.2117198119. PubMed Article
Vora SM, Fontana P, Mao T, Leger V, Zhang Y, Fu TM, Lieberman J, Gehrke L, Shi M, Wang L, Iwasaki A, Wu H
Proc Natl Acad Sci U S A. 2022 Mar 1;119(9). pii: 2117198119. doi: 10.1073/pnas.2117198119. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184637 | pLV-mScarlet-Control-5'UTR | Fluorescent reporter for mammalian cells: Expression of mScarlet with a CMV 5'UTR |
| 184638 | pLV-mScarlet-SARS-CoV-2-5'UTR | Fluorescent reporter for mammalian cells: Expression of mScarlet with SARS-CoV-2 5'UTR |
| 184639 | pLV-mScarlet-MAVS-5'UTR | Fluorescent reporter for mammalian cells: Expression of mScarlet with MAVS 5'UTR |
| 191480 | pLV-SARS-CoV-2 5'UTR-Luciferase | Luciferase reporter for SARS-CoV-2 5' UTR |
| 191483 | pCMV-Dest-MBP-Nsp1 | Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1 |
| 191484 | pLV-mScarlet-SARS-CoV-2-5'UTRΔSL1 | Lentiviral expression of SARS-CoV-2 5' UTR ΔSL1 |
| 191485 | pLV-mScarlet-SARS-CoV-2-SL1 alone | Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone |