Addgene: Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9.

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Jeffrey Bode
Akimoto et al

Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9.
Akimoto G, Fernandes AP, Bode JW
ACS Cent Sci. 2022 Feb 23;8(2):275-281. doi: 10.1021/acscentsci.1c01490. Epub 2022 Feb 9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
185755	pET-ChE1 v4.5	Bacterial expression of ChE1 v4.5
185756	pUb-HisUb-Ubc9-ChE1 v4.5	Bacterial expression of His-Ub, Ubc9 and ChE1 v4.5
185757	pSub-MBP-GFP(2M-IKQE)	Bacterial expression of MBP-GFP(2M IKQE)
185758	pSub-Asp6-MBP-HRas	Bacterial expression of Asp6-MBP-HRas
185759	pET28-His6-Ubc9-K14R	Bacterial expression of His6-Ubc9-K14R

Plasmids from Article

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