Long-term imaging of individual mRNA molecules in living cells.
Guo Y, Lee REC
Cell Rep Methods. 2022 May 25;2(6):100226. doi: 10.1016/j.crmeth.2022.100226. eCollection 2022 Jun 20.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185794 | cmv-sfGFP-GB1-scAB | scFv GFP compnent for SunRISER |
| 185795 | cmv-mCherry-8xPP7 | mCherry labeled with 8xPP7 |
| 185796 | cmv-mCherry-10xPP7 | mCherry labeled with 10xPP7 |
| 185797 | ubc-nls-pcp-5xSunTag (SRv1) | pcp-5xSunTag component for SunRISER SRv1 |
| 185798 | ubc-nls-pcp-10xSunTag (SRv1.1) | pcp-10xSunTag component for SunRISER SRv1.1 |
| 185799 | ubc-nls-pcp-12xSunTag (SRv1.2) | pcp-12xSunTag component for SunRISER SRv1.2 |
| 185800 | cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P) | one plasmid encoding scFv GFP and pcp-5xSunTag components for SunRISER SRv1 |
| 185801 | cmv-sfGFP-GB1-scAB-ubc-nls-pcp-10xSunTag(SRv1.1-2P) | one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.1 |
| 185802 | cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P) | one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2 |