Auxin-mediated induction of GAL promoters by conditional degradation of Mig1p improves sesquiterpene production in Saccharomyces cerevisiae with engineered acetyl-CoA synthesis.
Hayat IF, Plan M, Ebert BE, Dumsday G, Vickers CE, Peng B
Microb Biotechnol. 2021 Nov;14(6):2627-2642. doi: 10.1111/1751-7915.13880. Epub 2021 Sep 9. PubMed Article
Hayat IF, Plan M, Ebert BE, Dumsday G, Vickers CE, Peng B
Microb Biotechnol. 2021 Nov;14(6):2627-2642. doi: 10.1111/1751-7915.13880. Epub 2021 Sep 9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185861 | pJCble | The CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable marker |
| 185865 | pIALD2S | Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae |
| 185866 | pIALD2E | Knocking out ALD6 |
| 185867 | pIALD2HMGr | Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae |
| 185869 | pIPKAH | Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta) |