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Force generation of KIF1C is impaired by pathogenic mutations.
Siddiqui N, Roth D, Toleikis A, Zwetsloot AJ, Cross RA, Straube A
Curr Biol. 2022 Sep 12;32(17):3862-3870.e6. doi: 10.1016/j.cub.2022.07.029. Epub 2022 Aug 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
185978pFastBac-M13-6His-KIF1C_P176L-GFPBaculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.
185979pFastBac-M13-6His-KIF1C_R169W-GFPBaculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.
185980pKIF1C_P176L-GFPMammalian expression of human KIF1C with P176L mutation and GFP tag.
185981pKIF1C_R169W-GFPMammalian expression of human KIF1C with R169W mutation and GFP tag.
185982pKIF1C-mCherry-FRBMammalian expression of human KIF1C with mCherry and FRB tags.
185983pKIF1C_P176L-mCherry-FRBMammalian expression of human KIF1C with P176L mutation and mCherry and FRB tags.
185984pKIF1C_R169W-mCherry-FRBMammalian expression of human KIF1C with R169W mutation and mCherry and FRB tags.

Antibodies from Article