Addgene: Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex.

This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Browse
Jennifer Doudna
Wang et al

Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex.
Wang JY, Hoel CM, Al-Shayeb B, Banfield JF, Brohawn SG, Doudna JA
Nat Commun. 2021 May 6;12(1):2571. doi: 10.1038/s41467-021-22900-y. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
186274	pET_His6-MBP-TEV-Cas6-RT-Cas1_WT	Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli
186275	pET_His6-MBP-TEV-Cas2	Expresses Thiomicrospira Cas2 in E. coli
186276	pCRISPR	Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays
186277	pCRISPR_full-site	Reporter plasmid for full-site selection assay

Plasmids from Article

Antibodies from Article

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections

	More than 20 requests
	More than 50 requests
	More than 100 requests