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Analysis of the assembly pathway for membrane subunits of Complex I reveals that subunit L (ND5) can assemble last in E. coli.
Zhang F, Vik SB
BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2021.100027. Epub 2021 Oct 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
187084pBAD33(A-N)Medium expression of Complex I with subunit N-HA tagged
187086pBAD33(A-H*HA-N)Medium expression of Complex I with subunits N and H -HA tagged
187087pBAD33(EFG)Medium expression of subunits EFG

Antibodies from Article