Development of compact transcriptional effectors using high-throughput measurements in diverse contexts.
Tycko J, Van MV, A, DelRosso N, Yao D, Xu X, Ludwig C, Spees K, Liu K, Hess GT, Gu M, Mukund AX, Suzuki PH, Kamber RA, Qi LS, Bintu LB, Bassik MC.
bioRxiv. (PrePrint) Article
Tycko J, Van MV, A, DelRosso N, Yao D, Xu X, Ludwig C, Spees K, Liu K, Hess GT, Gu M, Mukund AX, Suzuki PH, Kamber RA, Qi LS, Bintu LB, Bassik MC.
bioRxiv. (PrePrint) Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187320 | pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast | dCas9 expression vector with cloning site for C terminal fusions |
| 187321 | pJT309 RapInducible1-ActivatorCloneSite-Citrine | Rapamycin inducible genetic circuit with cloning site for activator domains |
| 187324 | pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA | Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR |
| 187326 | pJT241 Lenti pEF-dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187327 | pJT242 Lenti pEF-dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187328 | pJT252 Lenti pEF-dCas9-3XFLAG-ZN473_TAD-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187329 | pJT253 Lenti pEF-dCas9-3XFLAG-FOXO3_TAD-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187330 | pJT254 Lenti pEF-dCas9-3XFLAG-NCOA3_TAD-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187331 | pJT255 Lenti pEF-dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187332 | pJT256 Lenti pEF-dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187333 | pJT257 Lenti pEF-dCas9-3XFLAG-Z+F-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187334 | pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187335 | pJT259 Lenti pEF-dCas9-3XFLAG-VP64-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187336 | pJT260 Lenti pEF-dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187337 | pJT282 Lenti pEF-dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187339 | pJT329 Lenti pEF-rTetR(SE-G72P)-3XFLAG-N+Z+F-T2A-mCherry-BSD-WPRE | rTetR effector fusion for manipulating transcription |
| 187341 | pJT337 Lenti pEF-dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187344 | pJT340 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187347 | pJT350 Lenti pEF-dCas9-3XFLAG-MBDa-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 187348 | pJT351 Lenti pEF-dCas9-3XFLAG-P66_CC-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 188743 | pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA | Donor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus |
| 188744 | pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA | Donor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus |
| 188745 | pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA | Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus |
| 188746 | pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA | Donor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus |
| 188747 | pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA | Donor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus |
| 188748 | pMV1 pEF1a-HGF | Vector constitutively expressing HGF |
| 188749 | pMV2 AAV-RapaInducible-NFZ-HGF | Rapamycin inducible AAV vector expressing HGF |
| 188750 | pMV4 lenti pEF-rTetR(SE-G72P)-3XFLAG-ANM2(SH3_1)-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing ANM2 SH3_1 domain as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188751 | pMV5 lenti pEF-rTetR(SE-G72P)-3XFLAG-APBB1(WW)-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing APBB1 WW domain as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188752 | pMV6 lenti pEF-rTetR(SE-G72P)-3XFLAG-CRTC2(TORC-C)-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing CRTC2 TORC_C domain as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188753 | pMV7 lenti pEF-rTetR(SE-G72P)-3XFLAG-CACO1(Zn-C2H2_12)-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing CACO1 Zn-C2H2_12 domain as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188754 | pMV8 lenti pEF-rTetR(SE-G72P)-3XFLAG-PYGO1(PHD)-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing PYGO1 PHD domain as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188755 | pMV9 lenti pEF-rTetR(SE-G72P)-3XFLAG-NOTC2(Notch)-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing NOTC2 Notch domain as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188756 | pMV10 lenti pEF-rTetR(SE-G72P)-3XFLAG-WWP2(WW)-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing WWP2 WW domain as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188757 | pJT151 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF10 KRAB-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing ZNF10 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188758 | pJT218 lenti pEF-rTetR(SE-G72P)-3XFLAG-VP64-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing VP64 as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188759 | PJT220 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF473 KRAB-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing ZNF473 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188760 | pJT222 lenti pEF-rTetR(SE-G72P)-3XFLAG-FOXO3 FOXO-TAD-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing FOXO3 FOXO-TAD as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188761 | pJT226 lenti pEF-rTetR(SE-G72P)-3XFLAG-SMARAC2 QLQ-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing SMARCA2 QLQ as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188762 | pJT228 lenti pEF-rTetR(SE-G72P)-3XFLAG-KIBRA WW-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing KIBRA WW as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188763 | pJT230 lenti pEF-rTetR(SE-G72P)-3XFLAG-NCOA3-TAD-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing NCOA3 as a fusion with rTetR(SE-G72P)-3XFLAG |
| 188764 | pJT236 lenti pEF-rTetR(SE-G72P)-3XFLAG-CXXC1 PHD-T2A-mCherry-BSD-WPRE | Lentiviral backbone containing CXXC1 PHD as a fusion with rTetR(SE-G72P)-3XFLAG |
| 195537 | pJT244 Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 195538 | pJT331 Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 195540 | pJT341 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 195541 | pJT342 Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 195542 | pJT300 Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast | dCas9 effector fusion for manipulating transcription |
| 195543 | pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD | dCas12a effector fusion for manipulating transcription |
| 195544 | pAR015 Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD | dCas12a effector fusion for manipulating transcription |
| 195545 | pAR028 Lenti pEF-dEnAsCas12a-NLS-ZNF705F KRAB-3XHA-T2A-BSD | dCas12a effector fusion for manipulating transcription |
| 195546 | pKL038 Lenti U6 dCas12a gRNA Puro mCherry | dCas12a gRNA expression backbone |
| 195547 | pAR013 Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD | dCas12a effector fusion for manipulating transcription |
| 195548 | pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD | dCas12a effector fusion for manipulating transcription |
| 195549 | pAR045 Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD | dCas12a effector fusion for manipulating transcription |
| 195550 | pAR012 Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD | dCas12a effector fusion for manipulating transcription |
| 202057 | pJT310 AAV-BFPdonor | AAV HDR donor |
| 202058 | pJT360 AAV-Rapamycin Inducible-noActivator Domain -Citrine | Inducible AAV with reporter gene and cloning site to add activator domains |
| 202059 | pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA | Inducible AAV activator |
| 202060 | pJT311 AAV-Rapamycin Inducible-NZF-Citrine | Inducible AAV activator |
| 202061 | pJT312 AAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator | Inducible AAV activator |
| 202062 | pJT313 AAV-Rapamycin Inducible-ZF-Citrine | Inducible AAV activator |
| 202063 | pJT314 AAV-Rapamycin Inducible-NZ-Citrine | Inducible AAV activator |
| 202064 | pJT315 AAV-Rapamycin Inducible-ZNF473KRAB-Citrine | Inducible AAV activator |
| 202066 | pJT365 AAV-Rapamycin Inducible-S3H | Inducible AAV activator |
| 202067 | pJT366 AAV-Rapamycin Inducible QNZF | Inducible AAV activator |