Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana.
Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio S, Orzaez D, Patron N
Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048. PubMed Article
Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio S, Orzaez D, Patron N
Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187560 | pEPKKα1KN0253 | Transcriptional unit for constitutive expression of CUP2:GAL4 driven by the nos promoter |
| 187561 | pEPCTΩSP0021 | Module for copper-inducible expression of firefly luciferase and nanoluc luciferase driven by minimal synthetic promoters with binding sites for CUP2 |
| 187562 | pEPCTΩSP0022 | Module for copper-inducible expression of nanoluc luciferase and firefly luciferase driven by minimal synthetic promoters with binding sites for CUP2 |
| 187563 | pEPCTαKN0009 | Transcriptional unit for copper-inducible expression of firefly luciferase driven by a minimal synthetic promoter with binding sites for CUP2 |
| 187564 | pEPCTαKN0013 | Transcriptional unit for copper-inducible expression of nanoluc luciferase driven by a minimal synthetic promoter with binding sites for CUP2 |
| 187565 | pEPCTΩSP0017 | Module for constitutive expression of firefly luciferase and nanoluc luciferase driven by 35s promoters |
| 187566 | pEPCTΩSP0018 | Module for constitutive expression of nanoluc luciferase and firefly luciferase driven by 35s promoters |
| 187568 | pEPCTαKN0001 | Transcriptional unit for constitutive expression of firefly luciferase driven by the 35s promoter |
| 187569 | pEPCTαKN0005 | Transcriptional unit for constitutive expression of nanoluc luciferase driven by the 35s promoter |
| 187570 | GB UA_114 A | Transcriptional unit for constitutive expression of Gal4:ΦC31 driven by the 35s promoter |
| 187571 | pEPKKα1RKN0115 | Transcriptional unit for constitutive expression of a TALE driven by the nos promoter |
| 187605 | pEPKKΩ1SP0678 | Module for copper inducible expression of AtrD11, HarFAR and EaDAct |
| 187803 | GB2513 | Module for constitutive expression of dCas9:EDLL, Ms2:VPR and a gRNA targeting the DFR promoter. |
| 187804 | GB1024 | Module for the constitutive expression of the delta-11-desaturase from Amyelois transitella and a fatty acid reductase from Helicoverpa armigera. |
| 187805 | GB1022 | Transcriptional unit for expression of diacylglycerol acetyltransferase from Euonymus alatus. |
| 187806 | GB3681 | Transcriptional unit for expression of alcohol O-acetyltransferase from Saccharomyces cerevisiae S288C, codon optimized for Nicotiana. |
| 187807 | GB3682 | Transcriptional unit for expression of alcohol O-acetyltransferase from Saccharomyces pastorianus strain CBS 1483 chromosome SeVIII-SeXV, codon optimized for Nicotiana. |
| 187808 | GB3683 | Transcriptional unit for expression of 1,2-diacyl-sn-glycerol:acetyl-CoA acetyltransferase from Euonymus fortunei, codon optimized for Nicotiana. |
| 187809 | GB3897 | Module for dCasEV2.1 activated expression of AtrD11, HarFAR and ScATF1 plus gRNA-1DFR. |
| 187810 | GB4068 | Module for the constitutive expression of Cup2:Gal4AD and copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR). |
| 187811 | GB4070 | Module for the constitutive expression of Cup2:Gal4AD and gRNA-1 DFR, and the copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR). |
| 187812 | GB3898 | Module for dCasEV2.1 activated expression of Atr delta11, HarFAR and ScATF1. |
| 187813 | GB4356 | Module for dCasEV2.1 activated expression of Atr delta11, HarFAR and SpATF1-2. |
| 187814 | GB4360 | Module for dCasEV2.1 activated expression ATF1, Atr delta11 and HarFAR. |
| 187815 | GB4361 | Module for dCasEV2.1 activated expression of ATF1-2, HarFAR and Atr delta11. |
| 187816 | GB4366 | Module for dCasEV2.1 activated expression of HarFAR, ATF1 and Atr delta11. |
| 187817 | GB4367 | Module for dCasEV2.1 activated expression for HarFAR, ATF1-2 and Atr delta11. |