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Live Cell Detection of Poly(ADP-Ribose) for Use in Genetic and Genotoxic Compound Screens.
Koczor CA, Haider AJ, Saville KM, Li J, Andrews JF, Beiser AV, Sobol RW
Cancers (Basel). 2022 Jul 28;14(15). pii: cancers14153676. doi: 10.3390/cancers14153676. PubMed Article

Plasmids from Article

ID Plasmid Purpose
187609pLV-EF1A-LivePAR(R163A)-HygroEGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.
187611pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-PuroSplit luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
187612pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-PuroSplit luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette

Antibodies from Article