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A kinesin-1 variant reveals motor-induced microtubule damage in cells.
Budaitis BG, Badieyan S, Yue Y, Blasius TL, Reinemann DN, Lang MJ, Cianfrocco MA, Verhey KJ
Curr Biol. 2022 Jun 6;32(11):2416-2429.e6. doi: 10.1016/j.cub.2022.04.020. Epub 2022 May 2. PubMed Article

Plasmids from Article

ID Plasmid Purpose
188071pN1-RnKIF5C(1-560)-Halo-FlagMammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags
188072pN1-RnKIF5C(1-560,Δ6)-Halo-FlagMammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.

Antibodies from Article