Improved loss-of-function CRISPR-Cas9 genome editing in human cells concomitant with inhibition of TGF-beta signaling.
Mishra T, Bhardwaj V, Ahuja N, Gadgil P, Ramdas P, Shukla S, Chande A
Mol Ther Nucleic Acids. 2022 Mar 8;28:202-218. doi: 10.1016/j.omtn.2022.03.003. eCollection 2022 Jun 14. PubMed Article
Mishra T, Bhardwaj V, Ahuja N, Gadgil P, Ramdas P, Shukla S, Chande A
Mol Ther Nucleic Acids. 2022 Mar 8;28:202-218. doi: 10.1016/j.omtn.2022.03.003. eCollection 2022 Jun 14. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188449 | pScalps eSpCas9_Zsgreen gRNA scaffold | Expression of eSpCas9 tagged with Zsgreen |
| 188450 | pTZ tRNA_gRNA empty scaffold | Expression of gRNAs using a human tRNA promoter |
| 188451 | pmGFP | Expression of a modified GFP (mGFP) with short half-life |