Addgene: Tunable Temperature-Sensitive Transcriptional Activation Based on Lambda Repressor.

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Mikhail Shapiro
Xiong et al

Tunable Temperature-Sensitive Transcriptional Activation Based on Lambda Repressor.
Xiong LL, Garrett MA, Buss MT, Kornfield JA, Shapiro MG
ACS Synth Biol. 2022 Jul 15;11(7):2518-2522. doi: 10.1021/acssynbio.2c00093. Epub 2022 Jun 15. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
195553	pCIwt-PRM-Wasabi	Always-on expression of mWasabi reporter with transcription activation by wildtype CI
195554	pTcI38-PRM-wasabi	Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38
195555	pTcI39-PRM-wasabi	Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39
195557	pTcI39-switch	Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1
195560	pBaseline-PRM-wasabi	Always-off, unactivated expression of mWasabi reporter

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