A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System.
Sohn J, Takahashi M, Okamoto S, Ishida Y, Furuta T, Hioki H
PLoS One. 2017 Jan 6;12(1):e0169611. doi: 10.1371/journal.pone.0169611. eCollection 2017. PubMed Article
Sohn J, Takahashi M, Okamoto S, Ishida Y, Furuta T, Hioki H
PLoS One. 2017 Jan 6;12(1):e0169611. doi: 10.1371/journal.pone.0169611. eCollection 2017. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190226 | pAAV2-DEST(f) | Gateway destination vector, empty AAV vector backbone |
| 190229 | pAAV2-SYN-GFP-BGHpA | Expresses EGFP in neuronal cells |
| 190230 | pENTR1A-SynTetOff-GFP | Gateway entry vector, high-level expression of EGFP in neuronal cells |
| 190231 | pAAV2-SynTetOff-GFP | AAV vector, high-level expression of EGFP in neuronal cells |
| 190232 | pAAV2-SynTetOff-palGFP | AAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cells |
| 190233 | pAAV2-SynTetOff-myrGFP | AAV vector, high-level expression of EGFP with a myristoylation/palmitoylation signal derived from Fyn N-terminus (myrGFP) in neuronal cells |
| 190234 | pAAV2-SynTetOff-FGL | AAV vector, high-level expression of myrGFP with a somatodendritic-targeting signal (FGL) in neuronal cells |
| 190236 | pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1] | AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase |
| 190238 | pAAV2-DEST(r) | Gateway destination vector, empty AAV vector backbone |
| 190239 | pAAV2-CMV-GFP-BGHpA | Expresses EGFP in mammalian cells |