Using fungible biosensors to evolve improved alkaloid biosyntheses.
d'Oelsnitz S, Kim W, Burkholder NT, Javanmardi K, Thyer R, Zhang Y, Alper HS, Ellington AD
Nat Chem Biol. 2022 Jul 7. pii: 10.1038/s41589-022-01072-w. doi: 10.1038/s41589-022-01072-w. PubMed Article
d'Oelsnitz S, Kim W, Burkholder NT, Javanmardi K, Thyer R, Zhang Y, Alper HS, Ellington AD
Nat Chem Biol. 2022 Jul 7. pii: 10.1038/s41589-022-01072-w. doi: 10.1038/s41589-022-01072-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190610 | pReg-RamR | Expresses the RamR transcription factor in E. coli |
| 190612 | pGfOMT1-GEN4 | Expresses an evolved methyltransferase for tetrahydropapaverine biosynthesis in E. coli |
| 190613 | pThpR | Expresses GFP in response to tetrahydropapaverine in E. coli |
| 190667 | pReg-GLAU4 | Expresses an evolved glaucine biosensor in E. coli |
| 190668 | pReg-PAP4 | Expresses an evolved papaverine biosensor in E. coli |
| 190669 | pReg-ROTU4 | Expresses an evolved rotundine biosensor in E. coli |
| 190670 | pReg-NOS4 | Expresses an evolved noscapine biosensor in E. coli |
| 190671 | pReg-THP4 | Expresses an evolved tetrahydropapaverine biosensor in E. coli |
| 194148 | Pramr-GFP | Contains a RamR-regulated promoter expressing GFP |
| 202631 | pSELIS-RamR | Enables dual growth and fluorescence based enrichment of RamR biosensors |