Luca Scorrano Lab:
Luca Scorrano plasmids
Scorrano L
unpublished
Scorrano L
unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191937 | pEGFP-N1-Letm1 | Expression of a Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1)- GFP chimera. |
| 191938 | pcDNA3.1-Bid-GFP | Expression of BID-GFP chimera |
| 191939 | pMSCV-YFP-Bak-mito | Exogenous expression of BAK-YFP chimera in mitochondria |
| 191940 | pcDNA3.1 Calreticulin | Expression of calreticulin |
| 191941 | pMSCVpuro-Bax-YFP | Exogenous expression of a Bax-YFP chimera. |
| 191942 | pECFP-Drp1 | Expression of a Drp1-ECFP chimera |
| 191943 | pcDNA3.1-HA-Bax-mito | Expression of Bax and mitochodrial targeting sequence. |
| 191944 | pcDNA3.1-HA-Bax | Expression of HA-Bax. |
| 191946 | pCI-His-Fis1-DTM | Expression of Fis1 lacking the C-terminal 27 residues (Fis1-DTM) |
| 191947 | pCI-HA-Fis1 | Expression of Fis1 |
| 191948 | pMSCV-YFP-Bak-ER | Expression of a YFP- Bak chimera and endoplasmatic reticulum signal peptide. |
| 191949 | pSilencer-Opa1 KO | Expression of a siRNA that targets Opa1 |
| 191950 | pEGFP-Fis1-DTM | Expression of a Fis1-GFP chimera lacking the C-terminal 27 residues (Fis1-DTM) |
| 191951 | pMSCVpuro-CFP-Bax | Expression of CFP-Bax chimera |
| 191952 | pMSCV-Opa1 | Exogenous expression of OPA1 |
| 191953 | pcB6-Myc-Mfn2 IYFFT | Expression of Mfn2 protein, in which the C-terminus, after the transmembrane domain, is replaced by a stretch of hydrophobic amino acids (IYFFT) |
| 191954 | pMIMY | A pMSCV-based backbone where the MCS has been substituted with a MCS-IRES-mitoYFP for expression of a gene of interest and a mitochondrially targeted YFP |
| 191955 | pcDNA3.1-Mfn1 | Expression of Mfn1, a mediator of mitochondrial fusion. |
| 191956 | pcDNA3.1-CnA-H151A | Expression of phosphatase dead mutant of calcineurin A (CnA-H151A). |
| 191957 | pcDNA3-PARL-D319N-FLAG C-t | Expression of Presenilin Associated Rhomboid Like (PARL) D319N mutant |
| 191958 | pcDNA3-PARL-D319A-FLAG C-t | Expression of Presenilin Associated Rhomboid Like (PARL) D319A mutant |
| 191959 | pMSCV-YFP-Bak | Expression of YFP-Bak chimera |
| 191960 | pcDNA3.1-Mfn2 | Expression of the mitochondrial GTPase Mfn2. |
| 191961 | pDEST-Opa1 | Gateway Destination Vector, containing OPA1 coding sequence, for Gateway Cloning Technology |
| 191962 | pcDNA3.1-Letm1 | Expression of Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1). |
| 191963 | pcDNA3.1-Opa1 | Expression of OPA1. |
| 191964 | pMSCVpuro-Bcl2 | Exogenous expression of Bcl2 WT |
| 191968 | pcDNA-DEST47-Noa1-GFP | Gateway Destination Vector, containing NOA1-GFP chimera coding sequence, for Gateway Cloning Technology |
| 191969 | pCDNA3.2/V5-DEST-Noa1 | Overexpression of Nitric Oxide Associated 1 protein (NOA1), tagged with V5. |
| 191970 | pcDNA3.1-p15 Bid-KKAA-GFP | Expression of the active p15 cBid KKAA mutant, chimera with GFP. |
| 191971 | pMSCV2.2-IRES- GFP-Opa1 | Plasmid for cloning reaction, containing OPA1 coding sequence. |
| 191972 | pEXP1-DEST-Noa1-HIS | Destination vector containing HIS-tagged NOA1 coding sequence. |
| 191980 | pMSCV-Mic60 | Expression of V5-tagged Mic60, a component of the MICOS complex. |
| 191981 | pcDNA3.1/Hygro-Mfn2-ActA | Expression of Mfn2 tagged with mitochondrion localization signal (ActA) |