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A genome-scale gain-of-function CRISPR screen in CD8 T cells identifies proline metabolism as a means to enhance CAR-T therapy.
Ye L, Park JJ, Peng L, Yang Q, Chow RD, Dong MB, Lam SZ, Guo J, Tang E, Zhang Y, Wang G, Dai X, Du Y, Kim HR, Cao H, Errami Y, Clark P, Bersenev A, Montgomery RR, Chen S
Cell Metab. 2022 Apr 5;34(4):595-614.e14. doi: 10.1016/j.cmet.2022.02.009. Epub 2022 Mar 10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
192187pSC014_pLKO-U6-BsmBI-MS2sgRNA-EFS-Thy11-2A-MS2-p65-HSF1TdgA vector
192188pLY57_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2-TRAC-RHA(3166mut)CD22 CAR AAV vector PRODH2 KI (pLY057)
192189pLY59_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2(stop)-TRAC-RHA(3166mut)CD22 CAR AAV vector PRODH2(StopCtrl) (pLY059)
192190pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)HER2 CAR AAV vector PRODH2 KI (pLY080b)
192191pLY088_AAV_EFS-BCMA-41BBz-PRODH2BCMA CAR AAV vector PRODH2 KI (pLY088)
192196pLY095-EFS-ERBB2 HER2-Blast-WPRELenti-HER2-OE-Blast
192203pXD023 - pLKO-EF1a-Puro-P2A-FLuc-WPRELenti-PL(Puro-Luciferase)
192204pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRELenti-GL(GFP-Luciferase)

Antibodies from Article