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Enhancing gene editing and gene targeting efficiencies in Arabidopsis thaliana by using an intron-containing version of ttLbCas12a.
Schindele P, Merker L, Schreiber T, Prange A, Tissier A, Puchta H
Plant Biotechnol J. 2023 Mar;21(3):457-459. doi: 10.1111/pbi.13964. Epub 2022 Nov 30. PubMed Article

Plasmids from Article

ID Plasmid Purpose
192350pAGT8162 (AATG_dttLbCas12a-i_TTCG)L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression
192351pAGT5439L0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stop
192352pAGT9403L0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stop
192353pAGT10446L0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stop
192453pAGT8163 (AATG_ttLbCas12a-i_TTCG)intronized ttLbCas12a for in planta expression

Antibodies from Article