Addgene: Rheb regulates nuclear mTORC1 activity independent of farnesylation.

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Jin Zhang
Zhong et al

Rheb regulates nuclear mTORC1 activity independent of farnesylation.
Zhong Y, Zhou X, Guan KL, Zhang J
Cell Chem Biol. 2022 Jun 16;29(6):1037-1045.e4. doi: 10.1016/j.chembiol.2022.02.006. Epub 2022 Mar 15. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
192436	pcDNA3-mCherry-Rheb	Encodes mCherry-tagged wild-type Rheb.
192437	pcDNA3-mCherry-Rheb(C181S)	Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.
192438	pcDNA3-Lyso-mCherry-Rheb(C181S)	Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.
192439	pcDNA3-mCherry-Rheb(5A)	Encodes mCherry-tagged Rheb lacking residues 38-42.
192440	pcDNA3-mCherry-TSC2	Encodes mCherry-tagged wild-type TSC2 isoform 5.
192441	pcDNA3-mCherry-TSC2-NLS	Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.
192442	pcDNA3-mCherry-TSC2(AA)	Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.
192443	pcDNA3-mCherry-TSC2(AA)-NLS	Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.
192444	pcDNA3-mCherry-TSC2(AA,3Q)	Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.
192445	pcDNA3-mCherry-TSC2(AA,3Q)-NLS	Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.

Antibodies from Article