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Stress-dependent cell stiffening by tardigrade tolerance proteins that reversibly form a filamentous network and gel.
Tanaka A, Nakano T, Watanabe K, Masuda K, Honda G, Kamata S, Yasui R, Kozuka-Hata H, Watanabe C, Chinen T, Kitagawa D, Sawai S, Oyama M, Yanagisawa M, Kunieda T
PLoS Biol. 2022 Sep 6;20(9):e3001780. doi: 10.1371/journal.pbio.3001780. eCollection 2022 Sep.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
192460pAcGFP1-N1-RvCAHS3-GSExpression of RvCAHS3-GS_linker-AcGFP1 in mammalian cells
192461pAcGFP1-N1-RvCAHS8-GSExpression of RvCAHS8-GS_linker-AcGFP1 in mammalian cells
192462pAcGFP1-N1-RvCAHS12-GSExpression of RvCAHS12-GS_linker-AcGFP1 in mammalian cells
192463pAcGFP1-N1-RvCAHS3-min-GSExpression of RvCAHS3-min-GS_linker-AcGFP1 in mammalian cells
192465pAcGFP1-N1-RvCAHS12-min-GSExpression of RvCAHS12-min-GS_linker-AcGFP1 in mammalian cells
192466pEThT-RvCAHS3Bacterial expression of RvCAHS3
192467pEThT-RvCAHS8Bacterial expression of RvCAHS8
192468pEThT-RvCAHS12Bacterial expression of RvCAHS12
192469pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1Transient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells
192470pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1Transient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells
192471pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1Transient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells
192472pAc5-STABLE2-neo-RvCAHS3Stable expression of RvCAHS3 (fly codon optimized) in Drosophila cells

Antibodies from Article