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Closely related type II-C Cas9 orthologs recognize diverse PAMs.
Wei J, Hou L, Liu J, Wang Z, Gao S, Qi T, Gao S, Sun S, Wang Y
Elife. 2022 Aug 12;11. pii: 77825. doi: 10.7554/eLife.77825.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
192143Nsp2Cas9_AAVExpresses Nsp2Cas9, and cloning backbone for sgRNA
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192144Nsp2-SmuCas9_AAVExpresses Nsp2-SmuCas9, and cloning backbone for sgRNA
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192145NarCas9_AAVExpresses NarCas9?and cloning backbone for sgRNA
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192146U6-Nme2_tracrExpresses Nme2-sgRNA cloned with BspQI
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Antibodies from Article