Generation and diversification of recombinant monoclonal antibodies.
DeLuca KF, Mick JE, Ide AH, Lima WC, Sherman L, Schaller KL, Anderson SM, Zhao N, Stasevich TJ, Varma D, Nilsson J, DeLuca JG
Elife. 2021 Dec 31;10. pii: 72093. doi: 10.7554/eLife.72093. PubMed Article
DeLuca KF, Mick JE, Ide AH, Lima WC, Sherman L, Schaller KL, Anderson SM, Zhao N, Stasevich TJ, Varma D, Nilsson J, DeLuca JG
Elife. 2021 Dec 31;10. pii: 72093. doi: 10.7554/eLife.72093. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193618 | pDL001_Hec1-ms_IgG_HC | Hec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells. |
| 193619 | pDL002_Hec1-ms_IgG_LC | Hec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells. |
| 193620 | pDL023_Tub-rb_IgG_HC | α-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells. |
| 193621 | pDL024_Tub-rb_IgG_LC | α-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells. |
| 193622 | pDL025_HA-rb_IgG_HC_15F11 | HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026. |
| 193623 | pDL026_HA-rb_IgG_LC_15F11 | HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025. |