Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors.
Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L
Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8. PubMed Article
Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L
Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193639 | pCMV-dCas12a-A3A-N57G | Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193640 | pCMV-dCas12a-A3A-Y130F | Expression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193641 | pCMV-dCas12a-A3A-Y132D | Expression of base editor dCas12a-A3A-Y132D in mammalian cells for base editing |
| 193642 | pCMV-dCas12a-A3B | Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193643 | pCMV-dCas12a-A3G | Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193644 | pCMV-dCas12a-ABE7 | Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193645 | pCMV-dCas12a-ABE8e | Expression of base editor dCas12a-ABE8e in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193646 | pCMV-dCas12a-ABE8e-V106W | Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193647 | pCMV-dCas12a-AID | Expression of base editor dCas12a-AID in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193648 | pCMV-dCas12a-BE | Expression of base editor dCas12a-BE in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193649 | pCMV-dCas12a-CDA1 | Expression of base editor dCas12a-CDA1 in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193650 | pCMV-dCas12a-eAID | Expression of base editor dCas12a-eAID in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193651 | pCMV-enCas12a-A3A-N57G | Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193652 | pCMV-enCas12a-A3A-Y130F | Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193653 | pCMV-enCas12a-ABE8e-V106W | Expression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193654 | pCMV-RR-A3A-N57G | Expression of base editor RR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193655 | pCMV-RR-A3A-Y130F | Expression of base editor RR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193656 | pCMV-RR-ABE8e-V106W | Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193657 | pCMV-RVR-A3A-N57G | Expression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193658 | pCMV-RVR-A3A-Y130F | Expression of base editor RVR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193659 | pCMV-RVR-ABE8e-V106W | Expression of base editor RVR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence |
| 193660 | U6-sgRNA-hcr3 | Expression of tandem pre-sgRNA array hcr3 for LbCas12a |
| 193661 | U6-sgRNA-hcr5 | Expression of tandem pre-sgRNA array hcr5 for LbCas12a |
| 193662 | U6-sgRNA-pcr4 | Expression of tandem pre-sgRNA array pcr4 for LbCas12a |