Multiplex profiling of developmental cis-regulatory elements with quantitative single-cell expression reporters.
Lalanne JB, Regalado SG, Domcke S, Calderon D, Martin BK, Li X, Li T, Suiter CC, Lee C, Trapnell C, Shendure J
Nat Methods. 2024 Jun;21(6):983-993. doi: 10.1038/s41592-024-02260-3. Epub 2024 May 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194097 | pre-associated oBC-mBC backbone (p025) | pre-associated oBC-mBC backbone (p025), can insert additional reporter constructs |
| 194098 | pre-associated oBC-minP-GFP-mBC backbone (p043) | pre-associated oBC-minP-GFP-mBC backbone (p043), can directly be used to deposit library of regulatory elements |
| 217420 | scQers promoter control #1 (low: minimal promoter, p027) | scQers promoter control #1 (low: minimal promoter, p027) |
| 217421 | scQers promoter control #2 (low: no promoter, p029) | scQers promoter control #2 (low: no promoter, p029) |
| 217422 | scQers promoter control #3 (intermediate: UBC promoter, p041) | scQers promoter control #3 (intermediate: UBC promoter, p041) |
| 217423 | scQers promoter control #4 (intermediate: Pgk1 promoter, p042) | scQers promoter control #4 (intermediate: Pgk1 promoter, p042) |
| 217424 | scQers promoter control #5 (high: EEF1A1 promoter, p028) | scQers promoter control #5 (high: EEF1A1 promoter, p028) |