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Multiplex profiling of developmental enhancers with quantitative, single-cell expression reporters

Lalanne JB, Regalado SG, Domcke S, Calderon D, Martin B, Li T, Suiter CC, Lee C, Trapnell C, Shendure J
bioRxiv 2022.12.10.519236 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
194097pre-associated oBC-mBC backbone (p025)pre-associated oBC-mBC backbone (p025), can insert additional reporter constructs
194098pre-associated oBC-minP-GFP-mBC backbone (p043)pre-associated oBC-minP-GFP-mBC backbone (p043), can directly be used to deposit library of regulatory elements
217420scQers promoter control #1 (low: minimal promoter, p027)scQers promoter control #1 (low: minimal promoter, p027)
217421scQers promoter control #2 (low: no promoter, p029)scQers promoter control #2 (low: no promoter, p029)
217422scQers promoter control #3 (intermediate: UBC promoter, p041)scQers promoter control #3 (intermediate: UBC promoter, p041)
217423scQers promoter control #4 (intermediate: Pgk1 promoter, p042)scQers promoter control #4 (intermediate: Pgk1 promoter, p042)
217424scQers promoter control #5 (high: EEF1A1 promoter, p028)scQers promoter control #5 (high: EEF1A1 promoter, p028)

Antibodies from Article