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Formation of Biomolecular Condensates in Bacteria by Tuning Protein Electrostatics.
Yeong V, Werth EG, Brown LM, Obermeyer AC
ACS Cent Sci. 2020 Dec 23;6(12):2301-2310. doi: 10.1021/acscentsci.0c01146. Epub 2020 Nov 12. PubMed Article

Plasmids from Article

ID Plasmid Purpose
199160pET-6xHis-GFP(+6)used to express a supercharged GFP variant in E. coli
199161pET-6xHis-GFP(+12)used to express a supercharged GFP variant in E. coli
199162pET-6xHis-GFP(+18)used to express a supercharged GFP variant in E. coli
199163pET-6xHis-GFP(+24)used to express a supercharged GFP variant in E. coli
199164pET-6xHis-GFP(+30)used to express a supercharged GFP variant in E. coli
199165pET-6xHis-GFP(+36)used to express a supercharged GFP variant in E. coli
199166pET-6xHis-GFP(0)used to express a neutral GFP variant in E. coli
199167pET-6xHis-GFP(-30)used to express a supercharged GFP variant in E. coli
199168pET-6xHis-GFP(+36)-DL4used to express a supercharged GFP variant that forms inclusion bodies in E. coli
199171pET-6xHis-sfGFPused to express a superfolder GFP in E. coli for comparison to supercharged variants

Antibodies from Article