Multiplex Genome Editing of Human Pluripotent Stem Cells Using Cpf1.
Ma H
Bio Protoc. 2024 Nov 20;14(22):e5108. doi: 10.21769/BioProtoc.5108. eCollection 2024 Nov 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194715 | CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector | Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sites |
| 194716 | CMV:AsCpf1-2A-GFP-U6-AAVS1-sg | Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hAAVS1 locus |
| 194717 | CMV:AsCpf1-2A-GFP-U6-GFP-sg | Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target GFP |
| 194718 | CMV:AsCpf1-2A-GFP-U6-MAFB-sg | Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hMAFB |
| 194719 | CMV:AsCpf1-2A-GFP-U6-INS-sg | Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINS |
| 194720 | pX458-MAFB-sg | px458 with guide RNA that target hMAFB |
| 194721 | pX458-AAVS1-sg | px458 with guide RNA that target hAAVS1 |
| 194722 | CMV:AsCpf1-2A-GFP-U6-tdTomato-sg | Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target tdTomato |
| 194723 | CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA | based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1 |
| 194724 | CAGGS-AsCpf1-2A-GFP-U6-tdTomato-sgRNA | based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target tdTomato |
| 194725 | CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA | based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB |
| 194726 | CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector | CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector |
| 194727 | CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNA | CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA array targeting hAAVS1. |
| 194728 | AAVS1-tdTomato targeting vector (compatible with CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA) | AAVS1-tdTomato targeting vector, compartible with Cpf1 |