A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing.
Wang Y, Qi T, Liu J, Yang Y, Wang Z, Wang Y, Wang T, Li M, Li M, Lu D, Chang ACY, Yang L, Gao S, Wang Y, Lan F
Sci Adv. 2023 Feb 10;9(6):eabo6405. doi: 10.1126/sciadv.abo6405. Epub 2023 Feb 10. PubMed Article
Wang Y, Qi T, Liu J, Yang Y, Wang Z, Wang Y, Wang T, Li M, Li M, Lu D, Chang ACY, Yang L, Gao S, Wang Y, Lan F
Sci Adv. 2023 Feb 10;9(6):eabo6405. doi: 10.1126/sciadv.abo6405. Epub 2023 Feb 10. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194965 | Cas12j-8-puro-crRNA | The plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene. |
| 194966 | Cas12j-8-puro | The plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene. |
| 194967 | Cas12j-8-crRNA | The plasmid expresses Cas12j-8-crRNA expression elements. |