Creb5 establishes the competence for Prg4 expression in articular cartilage.
Zhang CH, Gao Y, Jadhav U, Hung HH, Holton KM, Grodzinsky AJ, Shivdasani RA, Lassar AB
Commun Biol. 2021 Mar 12;4(1):332. doi: 10.1038/s42003-021-01857-0. PubMed Article
Zhang CH, Gao Y, Jadhav U, Hung HH, Holton KM, Grodzinsky AJ, Shivdasani RA, Lassar AB
Commun Biol. 2021 Mar 12;4(1):332. doi: 10.1038/s42003-021-01857-0. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195020 | Creb5 shRNA | Bovine Creb5 shRNA targeting the Creb5 3′UTR |
| 195021 | LentiCRISPRCreb5gRNA | Sequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domain |
| 195022 | pENTR-Creb5(HA) | Gateway vector containing HA-tagged Creb5 |
| 195023 | pInducer20-iCreb5(HA) | HA-tagged Creb5 in pInducer20 lentivirus destination vector |
| 195024 | pLenti-Creb5(HA) | HA-tagged Creb5 in pLenti CMV Puro DEST (w118-1) vector |
| 195025 | pENTR-Creb5(T59/T61A)(HA) | Gateway vector containing HA-tagged Creb5 with T59/T61A mutation |
| 195026 | pENTR-Creb5(T59/T61D)(HA) | Gateway vector containing HA-tagged Creb5 with T59/T61D mutation |
| 195027 | pInducer20-iCreb5(T59/T61A)(HA) | HA-tagged Creb5(T59/T61A) in pInducer20 lentivirus destination vector |
| 195028 | pInducer20-iCreb5(T59/T61D)(HA) | HA-tagged Creb5(T59/T61D) in pInducer20 lentivirus destination vector |
| 195029 | GAL4(1-147) | The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector |
| 195030 | GAL4-Creb5 (1- 508) | GAL4-Creb5 fusion construct (full length, 508aa) |
| 195031 | GAL4-Creb5 (1- 128) | GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa) |
| 195032 | GAL4-Creb5 (1- 128) T59/T61 A | GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with T59/T61 A mutation) |
| 195033 | GAL4-Creb5 (1- 128) C18/C23 S | GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation) |
| 195034 | GL4.10 Prg4 promoter-luciferase | Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector |
| 195035 | GL4.10 E2E1- Prg4 promoter-luciferase | Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector |
| 195036 | pGL4.10 E3E2E1-Prg4 promoter-luciferase | Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector |
| 195037 | pGL4.10 E4E3E2E1-Prg4 promoter-luciferase | Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector |