Cellular assays identify barriers impeding iron-sulfur enzyme activity in a non-native prokaryotic host.
D'Angelo F, Fernandez-Fueyo E, Garcia PS, Shomar H, Pelosse M, Manuel RR, Buke F, Liu S, van den Broek N, Duraffourg N, de Ram C, Pabst M, Bouveret E, Gribaldo S, Py B, Ollagnier de Choudens S, Barras F, Bokinsky G
Elife. 2022 Mar 4;11:e70936. doi: 10.7554/eLife.70936. PubMed Article
D'Angelo F, Fernandez-Fueyo E, Garcia PS, Shomar H, Pelosse M, Manuel RR, Buke F, Liu S, van den Broek N, Duraffourg N, de Ram C, Pabst M, Bouveret E, Gribaldo S, Py B, Ollagnier de Choudens S, Barras F, Bokinsky G
Elife. 2022 Mar 4;11:e70936. doi: 10.7554/eLife.70936. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195055 | pBbA5a-sufCDSUB | Expresses SUF operon from B. subtilis from IPTG-inducible promoter |
| 195056 | pBbA5a-sufABCDSE | Expresses SUF operon from E. coli from IPTG-inducible promoter |