Addgene: The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner.

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Anthony Bretscher
Garbett et al

The scaffolding protein EBP50 regulates microvillar assembly in a phosphorylation-dependent manner.
Garbett D, LaLonde DP, Bretscher A
J Cell Biol. 2010 Oct 18;191(2):397-413. doi: 10.1083/jcb.201004115. Epub 2010 Oct 11. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
195207	GFP-NHERF1 3xSA	Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A)
195208	GFP-NHERF1 3xSD	Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D)
195209	GFP-NHERF1 F355R	Mammalian expression vector for GFP tagged NHERF1. Ezrin interaction-deficient mutant
195210	GFP-NHERF1 PDZ1*	Mammalian expression vector for GFP tagged NHERF1. PDZ1 GYGF>GYAA motif mutant
195211	GFP-NHERF1 PDZ1/2*	Mammalian expression vector for GFP tagged NHERF1. Dual PDZ1/2 GYGF>GYAA motif mutant
195212	GFP-NHERF1 PDZ2*	Mammalian expression vector for GFP tagged NHERF1. PDZ2 GYGF>GYAA motif mutant
195213	GFP-NHERF1 WT	Mammalian expression vector for GFP tagged NHERF1.

Antibodies from Article