Changing colors and understanding: the use of mutant chromogenic protein and informational suppressor strains of Escherichia coli to explore the central dogma of molecular biology.
DeWolf S, Van den Bogaard M, Hart RB, Hartman S, Boury N, Phillips GJ
J Microbiol Biol Educ. 2023 Oct 25;24(3):e00094-23. doi: 10.1128/jmbe.00094-23. eCollection 2023 Dec. PubMed Article
DeWolf S, Van den Bogaard M, Hart RB, Hartman S, Boury N, Phillips GJ
J Microbiol Biol Educ. 2023 Oct 25;24(3):e00094-23. doi: 10.1128/jmbe.00094-23. eCollection 2023 Dec. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195855 | XA92+pRCPam | E. coli nonsense suppressor strain (glnX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows purple when CP is induced with rhamnose. |
| 195856 | XA101+pRCPam | E. coli nonsense suppressor strain (serU) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows salmon when CP is induced with rhamnose. |
| 195857 | XA103+pRCPam | E. coli nonsense suppressor strain (tyrT) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. No color when grown with or without rhamnose. |
| 195858 | XA106+pRCPam | E. coli nonsense suppressor strain (leuX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows magenta when CP is induced with rhamnose. |
| 195859 | CSH130+pRCPam | WT E. coli strain transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Serves as control for suppressor strains. No color when grown with or without rhamnose. |
| 195860 | CSH130+pRCP | WT E. coli strain transformed with plasmid carrying WT chromogenic protein (CP). Serves as control for suppressor strains. Grows purple when CP is induced with rhamnose. |
| 195861 | pRCPam | Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains. |
| 195862 | pRCP | Encodes chromogenic protein (CP). CP inducible with rhamnose. |