An integrase toolbox to record gene-expression during plant development.
Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5. PubMed Article
Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195885 | L0-T7 Empty target Tp901 | Level 0 Empty Tp901 target: attP_Tp901-BpiI-attB_Tp901 with golden gate linker in pBLUNT plasmid. |
| 195886 | L0-T3 mTurq-tUBQ10 | Level 0 mTurq-tUBQ10 for integrase target construction |
| 195887 | L0-T4 tRBSC_mScarlet | Level 0 tRBSC-mScarlet for integrase target construction |
| 195888 | L0-I38 BpiI-pUBQ10-BpiI | Level 0 pUBQ10 promoter between BpiI sites for target construction |
| 195889 | L0-T6 Empty target Bxb1 | Level 0 Empty Bxb1 target: attP_Bxb1-BpiI-attB_Bxb1 with golden gate linker in pBLUNT plasmid. |
| 195890 | L0-T1 Target PhiC31 | Level 0 PhiC31 target: attP_PhiC31-pUBQ10-attB_PhiC31 with golden gate linker in pBLUNT plasmid. |
| 195891 | L0-I2 pPP2AA3-mutated GG0-GG1 | Level 0 pPP2AA3 promoter with GG0 and GG1 golden gate linkers |
| 195892 | L0-I9 PhiC31 GG1-GG4 | Level 0 PhiC31 integrase with GG1 and GG4 golden gate linkers |
| 195893 | L0-I10 PhiC31 GG2-GG4 | Level 0 PhiC31 integrase with GG2 GG4 golden gate linkers |
| 195894 | L0-I11 PhiC31-NLS GG1-GG4 | Level 0 PhiC31 inegrase NLS with GG1 GG4 Golden gate linkers |
| 195895 | L0-I12 PhiC31-NLS GG2-GG4 | Level 0 PhiC31 inegrase NLS with GG2 GG4 Golden gate linkers |
| 195896 | L0-I14 DST GG4-GG5 | Level 0 DST (destabilization RNA tag: SAUR tag) with GG4 GG5 golden gate linkers |
| 195897 | L0-I15 tUBQ1 GG4-GG6 | Level 0 tUBQ1 terminator with GG4 GG6 golden gate linkers |
| 195898 | L0-I16 tUBQ1 GG5-GG6 | Level 0 tUBQ1 terminator with GG5 GG6 golden gate linkers |
| 195899 | L0-I18 Ub-Arg GG1-GG2 | Level 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkers |
| 195901 | L0-I23 Bxb1 GG1-GG4 | Level 0 Bxb1 integrase with GG1 and GG4 golden gate linkers |
| 195902 | L0-I26 pUBQ10 GG0-GG1 | Level 0 pUBQ10 promoter with GG0 and GG1 golden gate linkers |
| 195903 | L0-I32 pLBD16 GG0-GG1 | Level 0 pLBD16 promoter with GG0 and GG1 golden gate linkers |
| 195904 | L0-I35 p35S-XVE-pEstradiol GG0-GG1 | Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers) |
| 195905 | L0-I39 PhiC31-Intein GG1-GG4 | Level 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkers |
| 195906 | L0-I40 Intein-PhiC31 GG1-GG4 | Level 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkers |
| 195907 | L0-I42 Tp901 GG1-GG4 | Level 0 Tp901 integrase with GG1 and GG4 golden gate linkers |
| 195908 | pNL2080 pGATA23 GG0-GG1 | Level 0 pGATA23 promoter with GG0 and GG1 golden gate linkers |
| 195909 | pNL2114 pARF19 GG0-GG1 | Level 0 pARF19 promoter with GG0 and GG1 golden gate linkers |
| 195910 | pNL2119 pARF7 GG0-GG1 | Level 0 pARF7 promoter with GG0 and GG1 golden gate linkers |
| 195911 | L1_ARF7_1 | Level 1 PhiC31 cassette with LR dvp promoter: pARF7-PhiC31-tUBQ1 |
| 195912 | L1_GATA23_2 | Level 1 PhiC31 cassette with LR dvp promoter: pGATA23-PhiC31-NLS-tUBQ1 |
| 195913 | L1_GATA23_1 | Level 1 PhiC31 cassette with LR dvp promoter: pGATA23-PhiC31-tUBQ1 |
| 195914 | L1_ARF19_2 | Level 1 PhiC31 cassette with LR dvp promoter: pARF19-PhiC31-NLS-tUBQ1 |
| 195915 | L1_ARF19_1 | Level 1 PhiC31 cassette with LR dvp promoter: pARF19-PhiC31-tUBQ1 |
| 195916 | L1_ARF19_4 | Level 1 PhiC31 cassette with LR dvp promoter: pARF19-PhiC31-DST-tUBQ1 |
| 195917 | L1P1 | Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-tUBQ1 |
| 195918 | L1P2 | Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-tUBQ1 |
| 195919 | L1P4 | Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-DST-tUBQ1 |
| 195920 | L1P5 | Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-DST-tUBQ1 |
| 195921 | L1P16 | Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-tUBQ1 |
| 195922 | L1P17 | Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-NLS-tUBQ1 |
| 195923 | L1P18 | Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-DST-tUBQ1 |
| 195924 | L1P19 | Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-NLS-DST-tUBQ1 |
| 195925 | L1P22 | Level 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-NLS-tUBQ1 |
| 195926 | L1P23 | Level 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-tUBQ1 |
| 195927 | L1P24 | Level 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-NLS-DST-tUBQ1 |
| 195928 | L1P25 | Level 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-DST-tUBQ1 |
| 195929 | L1P27 | Level 1 PhiC31 cassette with constitutive promoter: p35S-UbArg-PhiC31NLS-tUBQ1 |
| 195930 | L1P32 | Level 1 PhiC31 cassette with LR dvp promoter: pLBD16-PhiC31-tUBQ1 |
| 195931 | L1P33 | Level 1 PhiC31 cassette with LR dvp promoter : pLBD16-PhiC31NLS-tUBQ1 |
| 195932 | L1P38 | Level 1 PhiC31 cassette with estradiol inducible promoter: p35S_XVE-PhiC31-tUBQ1 |
| 195933 | L1P46 | Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3:PhiC31:t35S |
| 195934 | L1P47 | Level 1 PhiC31 cassette with constitutive promoter : pPP2AA3:PhiC31-NLS:t35S |
| 195935 | L1P56 | Level 1 PhiC31 cassette with constitutive promoter : p35S:UbGln:PhiC31-NLS:tUBQ1 |
| 195936 | L1P59 | Level 1 PhiC31-intein(DnaE-Npu) cassette : p35S-PI-t35S |
| 195937 | L1P60 | Level 1 Intein(DnaE-Ssp)-PhiC31 cassette pUBQ10-IP-tUBQ1 |
| 195938 | L1P63 | PhiC31-intein(DnaE-Npu) and Intein(DnaE-Ssp)-PhiC31 : pUBQ10-PI-tUBQ1-p35S-IP-t35S |
| 195939 | L1P78 | Level 1 pARF19 reporter : pARF19-mScarlet-NLS-tRBCS |
| 195940 | L1P79 | Level 1 pGATA23 reporter : pGATA23-mScarlet-NLS-tRBCS |
| 195941 | L1P80 | Level 1 pLBD16 reporter : pLBD16-mScarlet-NLS-tRBCS |
| 195942 | L1P96 | Level 1 PhiC31 cassette with LR dvp promoter : pLBD16-PhiC31-DST-tUBQ1 |
| 195943 | L1P97 | Level 1 PhiC31 cassette with LR dvp promoter : pGATA23-PhiC31-DST-tUBQ1 |
| 195944 | L1B1 | Level 1 Bxb1 cassette with constitutive promoter : pPP2AA3-Bxb1-tUBQ1 |
| 195945 | T1P/pUBQ10(mTurq->mScarlet) | PhiC31 target with pUBQ10 switching from mTurq to mScarlet |
| 195946 | T1B/pUBQ10(mTurq->mScarlet) | Bxb1 target with pUBQ10 switching from mTurq to mScarlet |
| 195947 | T1T/pUBQ10(mTurq->mScarlet) | Tp901 target with pUBQ10 switching from mTurq to mScarlet |