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Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes.
Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, Allain V, Li Z, Marson A, Eyquem J, Wilson RC
Nat Biomed Eng. 2023 May;7(5):647-660. doi: 10.1038/s41551-023-01032-2. Epub 2023 Apr 25. PubMed Article

Plasmids from Article

ID Plasmid Purpose
196244pRM053 His-TEV-Cas9 triNLS 0CExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)
196245pRM049 His-TEV-Cas9 triNLS 2CExpression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)
196246pRM055 His-TEV-Cas9 6xNLS 0CExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

Antibodies from Article