Efficient CRISPR-Cas9 based cytosine base editors for phytopathogenic bacteria.
Li C, Wang L, Cseke LJ, Vasconcelos F, Huguet-Tapia JC, Gassmann W, Pauwels L, White FF, Dong H, Yang B
Commun Biol. 2023 Jan 17;6(1):56. doi: 10.1038/s42003-023-04451-8. PubMed Article
Li C, Wang L, Cseke LJ, Vasconcelos F, Huguet-Tapia JC, Gassmann W, Pauwels L, White FF, Dong H, Yang B
Commun Biol. 2023 Jan 17;6(1):56. doi: 10.1038/s42003-023-04451-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196247 | pHMattR3-attR4-GFP | pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene |
| 196248 | pEN-dCas9-CDA1-UGI | Plasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitor |
| 196249 | pEN-sacB | Plasmid containing the sacB gene |
| 196250 | pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2 | Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119 |
| 196251 | pTL-Bag1-gRNA1 | Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs |
| 196252 | pTL-Bag2-gRNA2 | Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs |
| 196253 | pTL-Bag2T-gRNA2 | Plasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAs |
| 196254 | pTL-Bag3-gRNA3 | Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs |
| 196255 | pTL-Bag4-gRNA4 | Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs |