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A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens.
Guna A, Page KR, Replogle JR, Esantsi TK, Wang ML, Weissman JS, Voorhees RM
bioRxiv. 2023 Jan 22:2023.01.22.525086. doi: 10.1101/2023.01.22.525086. Preprint. PubMed Article

Plasmids from Article

ID Plasmid Purpose
196280pJR152Cassette used in dual sgRNA library generation
197348mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFPTo allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.
197349mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFPTo allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2.

Antibodies from Article